A simple procedure is described for preparing lyso-GM1, a GM1 derivative that lacks the fatty acid moiety, starting from GM1 ganglioside using a one-pot reaction. Ganglioside deacylation was carried out in KOH/propan-1-ol in the absence of oxygen. The yield of lyso-GM1 under optimal conditions (6 h, 90-degrees-C, 0.2 N KOH, 1 mm GM1) was 54%. The chemical structure of lyso-GM1 was determined by H-1-NMR and FAB-MS analyses, thus proving that the acetamide groups of galactosamine and sialic acid units were not affected during the deacylation reaction.
|Titolo:||PREPARATION OF LYSO-GM1 (II(3)NEU5ACGGOSE(4)-LONG CHAIN BASES) BY A ONE-POT REACTION|
|Parole Chiave:||gangliosides; GM1; lyso-gangliosides|
|Settore Scientifico Disciplinare:||Settore BIO/10 - Biochimica|
|Data di pubblicazione:||1992|
|Appare nelle tipologie:||01 - Articolo su periodico|