GM1 ganglioside tritium-labeled at C-3 of sphingosine has been administered to rat cerebellar granule cells. Tritiated polysialylated gangliosides were observed in the cytosol of the cells, where they resulted in a higher amount after a short period of chase. This, together with the data showing an increase of the tritiated polysialylated gangliosides in the total particulate fraction in parallel to the prolonging of the chase period, suggests that cytosolic gangliosides could be a way of transporting neosynthesized gangliosides from the Golgi apparatus to the plasma membranes.

FORMATION OF TRITIUM-LABELED POLYSIALYLATED GANGLIOSIDES IN THE CYTOSOL OF RAT CEREBELLAR GRANULE CELLS IN CULTURE FOLLOWING ADMINISTRATION OF [H-3] GM1 GANGLIOSIDE / V. CHIGORNO, M. VALSECCHI, S. SONNINO, G. TETTAMANTI. - In: FEBS LETTERS. - ISSN 0014-5793. - 277:1-2(1990), pp. 164-166. [10.1016/0014-5793(90)80834-6]

FORMATION OF TRITIUM-LABELED POLYSIALYLATED GANGLIOSIDES IN THE CYTOSOL OF RAT CEREBELLAR GRANULE CELLS IN CULTURE FOLLOWING ADMINISTRATION OF [H-3] GM1 GANGLIOSIDE

V. Chigorno
Primo
;
M. Valsecchi
Secondo
;
S. Sonnino
Penultimo
;
G. Tettamanti
Ultimo
1990

Abstract

GM1 ganglioside tritium-labeled at C-3 of sphingosine has been administered to rat cerebellar granule cells. Tritiated polysialylated gangliosides were observed in the cytosol of the cells, where they resulted in a higher amount after a short period of chase. This, together with the data showing an increase of the tritiated polysialylated gangliosides in the total particulate fraction in parallel to the prolonging of the chase period, suggests that cytosolic gangliosides could be a way of transporting neosynthesized gangliosides from the Golgi apparatus to the plasma membranes.
Biosynthesis; Cytosol; Ganglioside; Transport
Settore BIO/10 - Biochimica
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/185817
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