The developmental profiles of the gangliosides and those of the fatty acids and long-chain bases of the total ganglioside mixture of the brain of chicken were followed from the 10th day of incubation to the 63rd posthatching day. One O-acetylated polysialoganglioside that seems specific of the earlier embryonic stage and up to 21 alkali-stable components could be recognized by high-resolution two-dimensional TLC procedures and quantified by computer-assisted two-dimensional TLC densitometry. Besides a number of gangliosides identified by co-chromatography with reference standards, 10 were of unidentified structure, and within these 4 did not belong to the gangliotetrahexosyl series. Throughout embryonic life, the ceramide portion of gangliosides was found to contain the long-chain base species with 18 carbons. Those with 20 carbons, ~10% of the total, were found to be present only after hatching.
FURTHER-STUDIES ON THE CHANGES OF CHICKEN BRAIN GANGLIOSIDES DURING PRENATAL AND POSTNATAL LIFE / S. SONNINO, R. BASSI, V. CHIGORNO, G. TETTAMANTI. - In: JOURNAL OF NEUROCHEMISTRY. - ISSN 0022-3042. - 54:5(1990), pp. 1653-1660.
FURTHER-STUDIES ON THE CHANGES OF CHICKEN BRAIN GANGLIOSIDES DURING PRENATAL AND POSTNATAL LIFE
S. SONNINOPrimo
;R. BASSISecondo
;V. CHIGORNOPenultimo
;G. TETTAMANTIUltimo
1990
Abstract
The developmental profiles of the gangliosides and those of the fatty acids and long-chain bases of the total ganglioside mixture of the brain of chicken were followed from the 10th day of incubation to the 63rd posthatching day. One O-acetylated polysialoganglioside that seems specific of the earlier embryonic stage and up to 21 alkali-stable components could be recognized by high-resolution two-dimensional TLC procedures and quantified by computer-assisted two-dimensional TLC densitometry. Besides a number of gangliosides identified by co-chromatography with reference standards, 10 were of unidentified structure, and within these 4 did not belong to the gangliotetrahexosyl series. Throughout embryonic life, the ceramide portion of gangliosides was found to contain the long-chain base species with 18 carbons. Those with 20 carbons, ~10% of the total, were found to be present only after hatching.Pubblicazioni consigliate
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