Ferene(TM) (Diagnostic Chemicals LTD, Canada) is used as the chromogenic agent in an improved direct method for determining serum iron. The colour reagent contains hydroxylamine, a reducing agent stable in solution, and a protein solubilizer to avoid deproteinization. The interference of serum copper is eliminated by complexation with thiourea. Reliability of the method has been established by a separate study of the reaction parameters, replicate analysis and recovery experiments. The results obtained by the proposed method correlate well with results of atomic absorption spectroscopy. A fully automated application is also proposed using a centrifugal analyzer with starter dispenser.
Improved direct determination of serum iron / Giuliani F, De Luca U, Melzi d'Eril GV, Moratti R.. - In: HAEMATOLOGICA. - ISSN 0390-6078. - 70:1(1985), pp. 6-10.
Improved direct determination of serum iron
Melzi d'Eril GV;
1985
Abstract
Ferene(TM) (Diagnostic Chemicals LTD, Canada) is used as the chromogenic agent in an improved direct method for determining serum iron. The colour reagent contains hydroxylamine, a reducing agent stable in solution, and a protein solubilizer to avoid deproteinization. The interference of serum copper is eliminated by complexation with thiourea. Reliability of the method has been established by a separate study of the reaction parameters, replicate analysis and recovery experiments. The results obtained by the proposed method correlate well with results of atomic absorption spectroscopy. A fully automated application is also proposed using a centrifugal analyzer with starter dispenser.
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