A simple and rapid method for the determination of brain γ aminobutyric acid is described. The tissue is homogenized in 0.1 M formic acid and the internal standard 5 amino valeric acid is added. After centrifugation, an aliquot of the supernatant is evaporated and reacted at room temperature with a mixture of hexamethyldisilazane, trimethylchlorosilane, and BSTFA in dry pyridine. After a short reaction time, a few microliters of the reaction mixture is directly analyzed by mass fragmentography. The sensitivity of the method allows measurement of GABA in micrograms of brain tissue.
|Titolo:||A simple and highly sensitive mass fragmentographic procedure for γ aminobutyric acid determinations|
CATTABENI, FLAMINIO NICOLA (Primo)
GALLI, CORRADO LODOVICO (Secondo)
|Settore Scientifico Disciplinare:||Settore BIO/14 - Farmacologia|
|Data di pubblicazione:||ago-1976|
|Digital Object Identifier (DOI):||10.1016/0003-2697(76)90499-1|
|Appare nelle tipologie:||01 - Articolo su periodico|