A simple and rapid method for the determination of brain γ aminobutyric acid is described. The tissue is homogenized in 0.1 M formic acid and the internal standard 5 amino valeric acid is added. After centrifugation, an aliquot of the supernatant is evaporated and reacted at room temperature with a mixture of hexamethyldisilazane, trimethylchlorosilane, and BSTFA in dry pyridine. After a short reaction time, a few microliters of the reaction mixture is directly analyzed by mass fragmentography. The sensitivity of the method allows measurement of GABA in micrograms of brain tissue.
A simple and highly sensitive mass fragmentographic procedure for γ aminobutyric acid determinations / F. Cattabeni, C.L. Galli, T. Eros. - In: ANALYTICAL BIOCHEMISTRY. - ISSN 0003-2697. - 72:1-2(1976 Aug), pp. 1-7. [10.1016/0003-2697(76)90499-1]
A simple and highly sensitive mass fragmentographic procedure for γ aminobutyric acid determinations
F. CattabeniPrimo
;C.L. GalliSecondo
;
1976
Abstract
A simple and rapid method for the determination of brain γ aminobutyric acid is described. The tissue is homogenized in 0.1 M formic acid and the internal standard 5 amino valeric acid is added. After centrifugation, an aliquot of the supernatant is evaporated and reacted at room temperature with a mixture of hexamethyldisilazane, trimethylchlorosilane, and BSTFA in dry pyridine. After a short reaction time, a few microliters of the reaction mixture is directly analyzed by mass fragmentography. The sensitivity of the method allows measurement of GABA in micrograms of brain tissue.
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