A new ganglioside, containing an alkali-labile linkage, was extracted from mouse brain and purified. It represents 3.6% of total lipid-bound sialic acid in the tissue and was obtained in pure form with a yield of about 35%. It contains sphingosine, glucose, galactose, N-acetylgalactosamine and sialic acid in the molar ratio 1:1:2:1:4 and, upon exhaustive sialidase treatment gives the monosialoganglioside GM1. Partial acid hydrolysis, methylation analysis, gas-liquid chromatography-mass spectrometry and chromium trioxide oxidation studies showed its basic neutral glycosphingolipid core to be ganglio-N-tetraose-ceramide. Three of the four sialic acid residues are N-acetylneuraminic acid and one, as shown by gas-liquid chromatography-mass spectrometry, is 9-O-acetyl,N-acetylneuraminic acid, which contains the alkali labile linkage. 9-O-acetyl,N-acetylneuraminic acid is α-ketosidically linked to position 8 of the N-acetylneuraminic acid residue bound to position 3 of the internal galactose. The other two N-acetylneuraminic acid residues form a disialosyl residue linked to position 3 of external galactose. The complete structure of the studied ganglioside is as follows: NeuAcα2-8NeuAcα2-3Galβ1-3GalNAcβ1-4(9-O-Ac-NeuAcaα2-8NeuAcα2-1′-N-acylsphingosine, and it can be considered as a derivative of the tetrasialoganglioside GQ1b.
Isolation and characterization of a tetrasialoganglioside from mouse brain, containing 9-O-acetyl,N-acetylneuraminic acid / V. CHIGORNO, S. SONNINO, R. GHIDONI, G. TETTAMANTI. - In: NEUROCHEMISTRY INTERNATIONAL. - ISSN 0197-0186. - 4:6(1982), pp. 531-539.
|Titolo:||Isolation and characterization of a tetrasialoganglioside from mouse brain, containing 9-O-acetyl,N-acetylneuraminic acid|
CHIGORNO, VANNA LUIGIA (Primo)
SONNINO, SANDRO (Secondo)
GHIDONI, RICCARDO (Penultimo)
TETTAMANTI, GUIDO (Ultimo)
|Settore Scientifico Disciplinare:||Settore BIO/10 - Biochimica|
|Data di pubblicazione:||1982|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/0197-0186(82)90042-0|
|Appare nelle tipologie:||01 - Articolo su periodico|