HIV-1 Immunogen is a gp120-depleted whole killed virus vaccine candidate formulated with Incomplete Freund's Adjuvant (HIV-IFA). We evaluated in a mouse model the immunogenicity of HIV-IFA by itself and when combined with HYB2055, an immunomodulatory oligonucleotide consisting of a novel DNA structure and synthetic CpR immunostimulatory motif, as an adjuvant. C57/BL6 mice were immunized with HIV-IFA alone or combined with HYB2055. Mice treated with HYB2055 or with PBS were used as controls. Compared to HIV-IFA alone, immunization with HIV-IFA and HYB2055 combination elicited strong production of HIV- and p24-specific IFNgamma, RANTES, MIP 1alpha, and MIP 1beta, as well as high titers of HIV- and p24-specific antibodies. Inclusion of HYB2055 also reduced levels of IL-5 produced by HIV-IFA alone. HYB2055 enhances the immunogenicity of HIV-IFA and shifts responses towards a type 1 cytokine profile. The immune enhancing effects of HYB2055 adjuvant were dose-dependent. These findings warrant clinical evaluation of the HIV-1 immunogen/HYB2055 candidate as a therapeutic vaccine for HIV-1 infected patients.
Immunization with gp120-depleted whole killed HIV immunogen and a second-generation CpG DNA elicits strong HIV-specific responses in mice / D. Trabattoni, A. Clivio, D.H. Bray, L. Bhagat, S. Beltrami, G. Maffeis, E. Cesana, P.E. Lowry, F. Lissoni, E.R. Kandimalla, T. Sullivan, S. Agrawal, R. Bartholomew, M. Clerici. - In: VACCINE. - ISSN 0264-410X. - 24:9(2006), pp. 1470-1477.
|Titolo:||Immunization with gp120-depleted whole killed HIV immunogen and a second-generation CpG DNA elicits strong HIV-specific responses in mice|
TRABATTONI, DARIA LUCIA (Primo)
CLIVIO, ALBERTO (Secondo)
CLERICI, MARIO SALVATORE (Ultimo)
|Parole Chiave:||CpG; Cytokines; HIV; Immunology; Immunomodulatory oligonucleotide; TLR9; Vaccine|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
|Data di pubblicazione:||2006|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.vaccine.2005.05.047|
|Appare nelle tipologie:||01 - Articolo su periodico|