Steroid 5α-reductase is a system of two isozymes (5αR-1 and 5αR-2), which is related to several human disease. The synthesis of a chemiluminescent probe (AZA-LU) useful for purification of 5αR is described. AZA-LU has a chemiluminescence detectable up to 10-15 M and is a good inhibitor (IC50 = 0.5 μM) for rat 5αR-1 expressed in transformed Saccharomyces cerevisiae yeast. Incubation of AZA-LU with yeast lysates, followed by SDS-PAGE, led to the detection of two luminescent protein regions with estimated molecular weights of 26 kDa and 21 kDa.
Synthesis of a chemiluminescent probe useful for the purification of steroid 5α-reductase / A. Guarna, A. Poletti, F. Catrambone, G. Danza, A. Marrucci, M. Serio, F. Celotti, L. Martini. - In: BIOORGANIC & MEDICINAL CHEMISTRY LETTERS. - ISSN 0960-894X. - 6:16(1996), pp. 1997-2002.
Synthesis of a chemiluminescent probe useful for the purification of steroid 5α-reductase
A. PolettiSecondo
;F. CelottiPenultimo
;L. MartiniUltimo
1996
Abstract
Steroid 5α-reductase is a system of two isozymes (5αR-1 and 5αR-2), which is related to several human disease. The synthesis of a chemiluminescent probe (AZA-LU) useful for purification of 5αR is described. AZA-LU has a chemiluminescence detectable up to 10-15 M and is a good inhibitor (IC50 = 0.5 μM) for rat 5αR-1 expressed in transformed Saccharomyces cerevisiae yeast. Incubation of AZA-LU with yeast lysates, followed by SDS-PAGE, led to the detection of two luminescent protein regions with estimated molecular weights of 26 kDa and 21 kDa.
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