Serotonin (5HT) containing 180 instead of 160 was found in brain after exposing rats to an atmosphere of 1802. The 180 incorporation appeared linear with time and 22% of the 5HT in striatum contained 180 after one hour of exposure. 180 entered 5HT by enzymatic reaction rather than by isotope exchange as treatment with p-chlorophenylalanine, to block tryptophan hydroxylase, prevented incorporation, while more 180-5HT than normal was found in brain after treatment with pargyline, to block monoamine oxidase. Our studies suggest that the incorporation of 1802 into brain 5HT might be a feasible approach for evaluating 5HT turnover in animals and with some modification might be applied in man.
|Titolo:||Incorporation of 18O2 into brain serotonin in vivo as a procedure for estimating turnover: a feasibility study in animals|
GALLI, CORRADO LODOVICO (Primo)
|Settore Scientifico Disciplinare:||Settore BIO/14 - Farmacologia|
|Data di pubblicazione:||feb-1978|
|Digital Object Identifier (DOI):||10.1016/0024-3205(78)90427-7|
|Appare nelle tipologie:||01 - Articolo su periodico|