The entire nucleotide sequence of human T-cell lymphotropic virus type II (HTLV-II) from a previously described isolate of patient NRA (HTLV-IINRA) was determined. Clones encoding the 5′ LTR and gag, pol, env and tax/rex open reading frames were subcloned and sequenced on both strands. The provirus consisted of 8957 nucleotides and showed 95.2% homology with the HTLV-IIMo prototype at the nucleotide level. Less than 5% amino acid variation between HTLV-IINRA and HTLV-IIMo was observed for coding regions. Although isolate HTLV-IINRA had an additional 25 amino acids at the 3′ end of the tax/rex, this region was 96% homologous with the 5′ end of HTLV-IIMo 3′ LTR. To further investigate HTLV-II variability, a portion of the env gp46 gene derived from 9 HTLV-II infected persons was amplified by polymerase chain reaction and sequenced. Sequenced was obtained for 320 nucleotides corresponding to HTLV-IIMo position 5291 to 5610. Isolates similar to the HTLV-IIMo and HTLV-IINRA prototypes were identified, and sequences were highly conserved.

Complete nucleotide sequence of HTLV-II isolate RNA: comparison of envelope sequence variation of HTLV-II isolates from U.S. blood donors and U.S. and Italian IV drug users / H. LEE, K.B. IDLER, P. SWANSON, J.J. APARICIO, K.K. KIN, J.P. LAX, M. NGUYEN, T. MANN, G. LECKIE, A. ZANETTI, G. MARINUCCI, I.Y. CHEN, J.D. ROSENBLATT. - In: VIROLOGY. - ISSN 0042-6822. - 196:1(1993), pp. 57-69.

Complete nucleotide sequence of HTLV-II isolate RNA: comparison of envelope sequence variation of HTLV-II isolates from U.S. blood donors and U.S. and Italian IV drug users

A. ZANETTI;
1993

Abstract

The entire nucleotide sequence of human T-cell lymphotropic virus type II (HTLV-II) from a previously described isolate of patient NRA (HTLV-IINRA) was determined. Clones encoding the 5′ LTR and gag, pol, env and tax/rex open reading frames were subcloned and sequenced on both strands. The provirus consisted of 8957 nucleotides and showed 95.2% homology with the HTLV-IIMo prototype at the nucleotide level. Less than 5% amino acid variation between HTLV-IINRA and HTLV-IIMo was observed for coding regions. Although isolate HTLV-IINRA had an additional 25 amino acids at the 3′ end of the tax/rex, this region was 96% homologous with the 5′ end of HTLV-IIMo 3′ LTR. To further investigate HTLV-II variability, a portion of the env gp46 gene derived from 9 HTLV-II infected persons was amplified by polymerase chain reaction and sequenced. Sequenced was obtained for 320 nucleotides corresponding to HTLV-IIMo position 5291 to 5610. Isolates similar to the HTLV-IIMo and HTLV-IINRA prototypes were identified, and sequences were highly conserved.
Settore MED/42 - Igiene Generale e Applicata
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/185511
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