Four phosphorylation sites have been identified in the chicken progesterone receptor. Two of these sites exhibit basal phosphorylation which is enhanced upon treatment with hormone and two of the sites are phosphorylated in response to hormone. Mutation of one of these hormone dependent sites, Ser530 to Ala530, causes a decrease in transcriptional activation at low concentrations of hormone, but the activity is unaffected at high concentrations. However, the hormone binding of the mutant is unaffected suggesting that phosphorylation of Ser530 plays a role in facilitating the response of the receptor to low concentrations of hormone. The chicken progesterone receptor can be activated by modulators of kinases in the absence of hormone. The finding that signals initiated by tyrosine phosphorylation (through treatment with EGF) or through the dopamine receptor suggests that there are multiple means of activating chicken progesterone receptor. In contrast, the human progesterone receptor does not exhibit ligand independent activation; however, its activity in the presence of the agonist R5020 is enhanced by treatment with 8-Br-cAMP, an activator of protein kinase A, and treatment with 8-Br-cAMP causes the antagonist, RU486, to act as an agonist.
Phosphorylation and progesterone receptor function / N.L. Weigel, W. Bai, Y. Zhang, C.A. Beck, D.P. Edwards, A. Poletti. - In: JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY. - ISSN 0960-0760. - 53:1-6(1995 Jun), pp. 509-514. ((Intervento presentato al 9. convegno International Congress on Hormonal Steroids tenutosi a Dallas nel 1994.
|Titolo:||Phosphorylation and progesterone receptor function|
POLETTI, ANGELO (Ultimo)
|Parole Chiave:||Animals ; Receptors, Progesterone ; Phosphorylation ; Humans ; Molecular Sequence Data ; Amino Acid Sequence ; Signal Transduction ; Ligands ; Structure-Activity Relationship|
|Settore Scientifico Disciplinare:||Settore BIO/13 - Biologia Applicata|
|Data di pubblicazione:||giu-1995|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/0960-0760(95)00098-K|
|Appare nelle tipologie:||01 - Articolo su periodico|