The reversibility of the isomerization of the Δ8 to Δ7 bond in the biosynthesis of cholesterol was reexamined. For this purpose [4 14C,9α 3H1]cholest 7 en 3β ol was incubated with the 10000 x g soluble fraction of rat liver homogenate both under anaerobic and aerobic conditions. Almost complete exchange of the 9α hydrogen with the medium was observed under anaerobic conditions, whereas under aerobic conditions 34% exchange with the medium was calculated from the 3H/14C ratio found in cholesterol isolated after incubation. The stereospecificity of the Δ7 to Δ8 isomerization was established by incubating in analogous conditions [4 14C,7 3H1]cholest 7 en 3 β ol. The synthesis of each labeled substrate is described.
The reversibility of the isomerization of the Δ8 to Δ7 bond in cholesterol biosynthesis / A. Scala, M. Galli Kienle, M. Anastasia, G. Galli. - In: EUROPEAN JOURNAL OF BIOCHEMISTRY. - ISSN 0014-2956. - 48:1(1974), pp. 263-269.
|Titolo:||The reversibility of the isomerization of the Δ8 to Δ7 bond in cholesterol biosynthesis|
ANASTASIA, MARIO (Penultimo)
|Parole Chiave:||cholesterol ; carbon ; tritium, in vitro study ; theoretical study ; aerobic metabolism ; anaerobic growth ; animal ; article ; biosynthesis ; cell free system ; isomerism ; isotope labeling ; liver ; metabolism ; rat ; thin layer chromatography ; Aerobiosis ; Carbon Radioisotopes ; Cell-Free System ;|
|Settore Scientifico Disciplinare:||Settore BIO/10 - Biochimica|
|Data di pubblicazione:||1974|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1111/j.1432-1033.1974.tb03764.x|
|Appare nelle tipologie:||01 - Articolo su periodico|