The receptor tyrosine kinase RET, with a known role in embryonic development and in human pathologies, is alternatively spliced to yield at least two functional isoforms, which differ only in their carboxyl terminal. Enigma protein is a member of the PDZ-LIM family and is known to interact with the short isoform of RET/PTC2, a chimeric oncoprotein isolated from papillary thyroid carcinoma. Here, we show that Enigma also interacts in intact cells with the short isoform of RET-wt and of its pathologic mutants associated to MEN2 syndromes, RET-C634R and RET-M918T. In contrast, Enigma binds all the corresponding RET long isoforms very poorly and colocalizes with short but not long RET/PTC2 isoforms. The RET docking tyrosine for Enigma is the last but one before the divergence between the two isoforms and we demonstrated that short-isoform-specific amino acid residues +2 to +4 to this tyrosine are required for the interaction of RET/PTC2 with Enigma.
Differential interaction of Enigma protein with the two RET isoforms / M. G. Borrello, E. Mercalli, C. Perego, D. Degl'Innocenti, S. Ghizzoni, E. Arighi, B. Eroini, M. G. Rizzetti, M. A. Pierotti. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - 296:3(2002 Aug 23), pp. 515-22-522.
Titolo: | Differential interaction of Enigma protein with the two RET isoforms |
Autori: | |
Parole Chiave: | Enigma; Oncoproteins; RET; RET isoforms; Tyrosine kinase |
Settore Scientifico Disciplinare: | Settore BIO/09 - Fisiologia |
Data di pubblicazione: | 23-ago-2002 |
Rivista: | |
URL: | http://www.sciencedirect.com/science/article/pii/S0006291X02008860 |
Tipologia: | Article (author) |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/S0006-291X(02)00886-0 |
Appare nelle tipologie: | 01 - Articolo su periodico |