To investigate the role of complement activation in the adult respiratory distress syndrome (ARDS) and in the complications of extracorporeal circulation (ECC), several parameters (CH50, C3 split products, C3a, C5a, PMN aggregating activity, carboxypeptidase activity) of the complement profiles of 23 ARDS patients were measured. Twenty patients were treated by long-term extracorporeal support. Before connection to ECC, marked leukocytosis (18,250 +/- 5,950) and significantly high plasma C3a levels (p less than 0.005) were observed. After connection, C3a levels increased further, up to values eight times higher than the basal ones. The WBC count transiently decreased to 41% of prebypass levels after 15 minutes of ECC. At the same time C3 split products appeared and PMN aggregating activity was shown in 52% of the patients. C5a levels remained normal during bypass, even in two samples in which PMN aggregating activity was detected. Later decreases in CH50 titers (p less than 0.001) and carboxypeptidase activity (p less than 0.005) were observed. Complement activation was no longer evident after the 24th hour of bypass. We conclude that there is a low-degree complement activation in ARDS, and ECC is a further strong stimulus for complement activation. This phenomenon appears, however, to be self-limited.

Studies of complement activation in ARDS patients treated by long-term extracorporeal CO2 removal / M. Gardinali, M. Cicardi, D. Frangi, L. Bergamaschini, M. Gallazzi, L. Gattinoni, A. Agostoni. - In: INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS. - ISSN 0391-3988. - 8:3(1985 May), pp. 135-40-140.

Studies of complement activation in ARDS patients treated by long-term extracorporeal CO2 removal

M. Cicardi;L. Bergamaschini;L. Gattinoni;
1985-05

Abstract

To investigate the role of complement activation in the adult respiratory distress syndrome (ARDS) and in the complications of extracorporeal circulation (ECC), several parameters (CH50, C3 split products, C3a, C5a, PMN aggregating activity, carboxypeptidase activity) of the complement profiles of 23 ARDS patients were measured. Twenty patients were treated by long-term extracorporeal support. Before connection to ECC, marked leukocytosis (18,250 +/- 5,950) and significantly high plasma C3a levels (p less than 0.005) were observed. After connection, C3a levels increased further, up to values eight times higher than the basal ones. The WBC count transiently decreased to 41% of prebypass levels after 15 minutes of ECC. At the same time C3 split products appeared and PMN aggregating activity was shown in 52% of the patients. C5a levels remained normal during bypass, even in two samples in which PMN aggregating activity was detected. Later decreases in CH50 titers (p less than 0.001) and carboxypeptidase activity (p less than 0.005) were observed. Complement activation was no longer evident after the 24th hour of bypass. We conclude that there is a low-degree complement activation in ARDS, and ECC is a further strong stimulus for complement activation. This phenomenon appears, however, to be self-limited.
Humans; Respiratory Distress Syndrome, Adult; Complement C5; Respiration, Artificial; Child; Carboxypeptidases; Leukocyte Count; Complement C3; Complement C3a; Extracorporeal Circulation; Adult; Complement C5a; Middle Aged; Adolescent; Time Factors; Complement System Proteins; Male; Female; Complement Activation; Carbon Dioxide
Settore MED/41 - Anestesiologia
Settore MED/09 - Medicina Interna
INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/184788
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