Using an immunoblot technique we found a significantly higher frequency of serum IgG antibodies to a 35-kDa peripheral nerve myelin glycoprotein in patients with motor neuron disease (MND) (39% of 70) than in patients with neuropathy (13% of 61), other neurological disease (9% of 32) and normal subjects (5% of 20) (P < 0.005 in all cases), but not with multiple sclerosis (MS) (20% of 30) or non-neural immune diseases (25% of 32). Most positive patients had antibody titers of 1:200 or 1:2000 while higher titers were only found in seven patients with MND, one with chronic inflammatory demyelinating neuropathy, two with MS, two with non-neural immune diseases and one with stroke. The reacting protein had a higher molecular mass than P0 and was only faintly bound by an anti-P0 antiserum, but had the same N-terminal amino acid sequence of P0. The difference in molecular mass between P0 and the 35-kDa protein and the IgG reactivity of one patient's IgG with the 35-kDa protein persisted after its deglycosylation and dephosphorylation. Although there is no evidence that these antibodies are pathogenic, their frequent occurrence in MND and other immune-mediated conditions supports the hypothesis of an activation of the immune system in MND.
Serum IgG antibodies to a 35-kDa P0-related glycoprotein in motor neuron disease / E. Nobile-Orazio, E. Manfredini, M. Sgarzi, G. Spagnol, S. Allaria, M. Quadroni, G. Scarlato. - In: JOURNAL OF NEUROIMMUNOLOGY. - ISSN 0165-5728. - 53:2(1994 Sep), pp. 143-51-151.
|Titolo:||Serum IgG antibodies to a 35-kDa P0-related glycoprotein in motor neuron disease|
NOBILE-ORAZIO, EDUARDO (Primo)
SCARLATO, GUGLIELMO (Ultimo)
|Parole Chiave:||Antibodies; Myelin Proteins; Humans; Nervous System Diseases; Immunoglobulin G; Motor Neuron Disease|
|Settore Scientifico Disciplinare:||Settore MED/26 - Neurologia|
|Data di pubblicazione:||set-1994|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/0165-5728(94)90024-8|
|Appare nelle tipologie:||01 - Articolo su periodico|