IgM M-proteins in some motor neuron disease (MND) patients bind immunologically to shared determinants on gangliosides GM1 and GD1b. Since patients with these M-proteins have improved with immunotherapy the antibodies may be important in the pathogenesis of MND. To study how the M-proteins might damage motor neurons, we established co-cultures of human neurons from spinal cord explants and human myotubes. Antibodies from patient but not control serum bound to the cultured neurons. Neurons in co-cultures degenerated after incubation with patient but not control serum. These results demonstrate that anti-GM1 antibodies can bind to and destroy spinal cord neurons that are cultured with muscle. Nerve-muscle co-cultures can serve as a system to examine effects of anti-GM1/GD1b M-proteins on motor neurons.
|Titolo:||Anti-GM1/GD1b M-proteins damage human spinal cord neurons co-cultured with muscle|
|Parole Chiave:||Fetus; Spinal Cord; Humans; Muscles; Gangliosides; Binding Sites; Paraproteins; Cells, Cultured; Neurons; Carbohydrate Sequence; Molecular Sequence Data; Cell Communication; G(M1) Ganglioside; Middle Aged; Motor Neuron Disease; Immunoglobulin M|
|Settore Scientifico Disciplinare:||Settore MED/26 - Neurologia|
|Data di pubblicazione:||1-dic-1993|
|Digital Object Identifier (DOI):||10.1016/0022-510X(93)90022-Q|
|Appare nelle tipologie:||01 - Articolo su periodico|