Anti-GM1/GD1b M-proteins damage human spinal cord neurons co-cultured with muscle

Heiman Patterson, T.; Krupa, T.; Thompson, P.; Nobile-Orazio, E.; Tahmoush, A.J.; Shy, M.E.

doi:10.1016/0022-510X(93)90022-Q

IgM M-proteins in some motor neuron disease (MND) patients bind immunologically to shared determinants on gangliosides GM1 and GD1b. Since patients with these M-proteins have improved with immunotherapy the antibodies may be important in the pathogenesis of MND. To study how the M-proteins might damage motor neurons, we established co-cultures of human neurons from spinal cord explants and human myotubes. Antibodies from patient but not control serum bound to the cultured neurons. Neurons in co-cultures degenerated after incubation with patient but not control serum. These results demonstrate that anti-GM1 antibodies can bind to and destroy spinal cord neurons that are cultured with muscle. Nerve-muscle co-cultures can serve as a system to examine effects of anti-GM1/GD1b M-proteins on motor neurons.

Anti-GM1/GD1b M-proteins damage human spinal cord neurons co-cultured with muscle / T. Heiman-Patterson, T. Krupa, P. Thompson, E. Nobile-Orazio, A. J. Tahmoush, M. E. Shy. - In: JOURNAL OF THE NEUROLOGICAL SCIENCES. - ISSN 0022-510X. - 120:1(1993 Dec 01), pp. 38-45-45.

Titolo:	Anti-GM1/GD1b M-proteins damage human spinal cord neurons co-cultured with muscle
Autori:	T. Heiman Patterson T. Krupa P. Thompson NOBILE ORAZIO, EDUARDO A. J. Tahmoush M. E. Shy mostra contributor esterni nascondi contributor esterni
Parole Chiave:	Fetus; Spinal Cord; Humans; Muscles; Gangliosides; Binding Sites; Paraproteins; Cells, Cultured; Neurons; Carbohydrate Sequence; Molecular Sequence Data; Cell Communication; G(M1) Ganglioside; Middle Aged; Motor Neuron Disease; Immunoglobulin M
Settore Scientifico Disciplinare:	Settore MED/26 - Neurologia
Data di pubblicazione:	1-dic-1993
Rivista:	JOURNAL OF THE NEUROLOGICAL SCIENCES
Tipologia:	Article (author)
Digital Object Identifier (DOI):	http://dx.doi.org/10.1016/0022-510X(93)90022-Q
Appare nelle tipologie:	01 - Articolo su periodico

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