IgM M-proteins in some motor neuron disease (MND) patients bind immunologically to shared determinants on gangliosides GM1 and GD1b. Since patients with these M-proteins have improved with immunotherapy the antibodies may be important in the pathogenesis of MND. To study how the M-proteins might damage motor neurons, we established co-cultures of human neurons from spinal cord explants and human myotubes. Antibodies from patient but not control serum bound to the cultured neurons. Neurons in co-cultures degenerated after incubation with patient but not control serum. These results demonstrate that anti-GM1 antibodies can bind to and destroy spinal cord neurons that are cultured with muscle. Nerve-muscle co-cultures can serve as a system to examine effects of anti-GM1/GD1b M-proteins on motor neurons.
Anti-GM1/GD1b M-proteins damage human spinal cord neurons co-cultured with muscle / T. Heiman-Patterson, T. Krupa, P. Thompson, E. Nobile-Orazio, A. J. Tahmoush, M. E. Shy. - In: JOURNAL OF THE NEUROLOGICAL SCIENCES. - ISSN 0022-510X. - 120:1(1993 Dec 01), pp. 38-45-45.
Titolo: | Anti-GM1/GD1b M-proteins damage human spinal cord neurons co-cultured with muscle |
Autori: | |
Parole Chiave: | Fetus; Spinal Cord; Humans; Muscles; Gangliosides; Binding Sites; Paraproteins; Cells, Cultured; Neurons; Carbohydrate Sequence; Molecular Sequence Data; Cell Communication; G(M1) Ganglioside; Middle Aged; Motor Neuron Disease; Immunoglobulin M |
Settore Scientifico Disciplinare: | Settore MED/26 - Neurologia |
Data di pubblicazione: | 1-dic-1993 |
Rivista: | |
Tipologia: | Article (author) |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/0022-510X(93)90022-Q |
Appare nelle tipologie: | 01 - Articolo su periodico |