Endothelial cells undergo apoptosis after withdrawal of growth factors, alterations in the extracellular matrix, or exposure to cytokines. Here we report that tumor necrosis factor (TNF)-alpha induces apoptosis of human endothelial cells derived from the umbilical vein in a dose-dependent fashion. Apoptosis is triggered through a pathway that is independent from the levels of Bcl-2. On the contrary, TNF stimulates the growth of spontaneously transformed human umbilical vein endothelial cells. This proliferative effect is mediated through the up-regulation of fibroblast growth factor-1 by TNF. The addition of specific fibroblast growth factor-1 antisense oligonucleotides inhibits TNF-induced fibroblast growth factor-1 expression, thus inhibiting the growth and triggering apoptosis of spontaneously transformed human umbilical vein endothelial cells.
|Titolo:||Tumor-necrosis-factor-induced fibroblast growth factor-1 acts as a survival factor in a transformed endothelial cell line|
MAIER, JEANETTE ANNE MARIE (Primo)
BALSARI, ANDREA (Ultimo)
|Parole Chiave:||Tumor Necrosis Factor-alpha; Apoptosis; Oligodeoxyribonucleotides; Recombinant Proteins; Dose-Response Relationship, Drug; Oligonucleotides, Antisense; Humans; Endothelium, Vascular; Cell Survival; Fibroblast Growth Factor 2; Proto-Oncogene Proteins c-bcl-2; Base Sequence; Proto-Oncogene Proteins; Umbilical Veins; Molecular Sequence Data; Up-Regulation; Cell Line, Transformed; Cell Division|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
|Data di pubblicazione:||set-1996|
|Appare nelle tipologie:||01 - Articolo su periodico|