The HindIII c-sis RFLP was analyzed in constitutional and tumor DNAs of 19 meningioma patients. Loss of a c-sis allele (allele I in all cases) was observed in 6 out of 10 tumors from heterozygous patients. No alteration in the structure or dosage of the c-sis gene was detected in any tumor sample. Northern analysis of c-sis evidenced sis transcripts in 7 out of 8 meningiomas which were amenable to analysis. All tumors expressing c-sis also expressed c-fos, suggesting that the autocrine loop elicited by sis activation may have c-fos as its nuclear target. Co-expression of c-sis and c-fos was also observed in 5 neuromas out of 7 analyzed. Very high levels of fos-mRNA were observed in 2 neuromas, but were not found to be caused by apparent alteration or amplification of the c-fos gene.

Expression of c-sis and c-fos genes in human meningiomas and neurinomas / P. Riva, L. Larizza. - In: INTERNATIONAL JOURNAL OF CANCER. - ISSN 0020-7136. - 51:6(1992 Jul 30), pp. 873-877.

Expression of c-sis and c-fos genes in human meningiomas and neurinomas

P. Riva
Primo
;
L. Larizza
Ultimo
1992

Abstract

The HindIII c-sis RFLP was analyzed in constitutional and tumor DNAs of 19 meningioma patients. Loss of a c-sis allele (allele I in all cases) was observed in 6 out of 10 tumors from heterozygous patients. No alteration in the structure or dosage of the c-sis gene was detected in any tumor sample. Northern analysis of c-sis evidenced sis transcripts in 7 out of 8 meningiomas which were amenable to analysis. All tumors expressing c-sis also expressed c-fos, suggesting that the autocrine loop elicited by sis activation may have c-fos as its nuclear target. Co-expression of c-sis and c-fos was also observed in 5 neuromas out of 7 analyzed. Very high levels of fos-mRNA were observed in 2 neuromas, but were not found to be caused by apparent alteration or amplification of the c-fos gene.
RNA, Neoplasm; Platelet-Derived Growth Factor; Meningeal Neoplasms; Neuroma, Acoustic; Humans; Neurilemmoma; Aged; Proto-Oncogenes; Proto-Oncogene Proteins c-sis; DNA, Neoplasm; Deoxyribonuclease HindIII; Proto-Oncogene Proteins; Polymorphism, Restriction Fragment Length; Blotting, Southern; Adult; Spinal Cord Neoplasms; Middle Aged; Meningioma; Genes, fos; Adolescent; Female; Male
Settore MED/03 - Genetica Medica
Settore BIO/13 - Biologia Applicata
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/184353
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