The murine monoclonal antibody (MAb) MOv2 was found to be directed against the carbohydrate moieties of different kinds of molecules expressed on a human ovarian cystoadenocarcinoma. To define further the glycoconjugates carrying the MOv2-defined epitope, different procedures were used to analyze materials from surgical specimens and carcinoma cell lines. SDS-PAGE and immunoblotting showed glycoprotein molecules migrating in the gel as high and intermediate molecular weight components. A low-molecular-weight band, migrating approximately with the dye front, was also immunostained by MOv2. On the other hand, the immunostaining of high-performance thinlayer chromatography (HPTLC) of the total glycolipid extract and its neutral and acid fractions, after DEAE chromatography, showed selective reactivity with a neutral glycolipid. Reanalysis by immunoblotting of this glycolipid band scraped off the HPTLC plate indicated that it corresponds to the low-molecular-weight component. Periodate oxidation and Pronase digestion further demonstrate the saccharide nature of the determinant on both types of glycoconjugates. In conclusion, we report evidence that with a single analytical procedure, i.e., immunoblotting, it is possible to recognize the same carbohydrate determinant carried on both protein and lipid molecules.
CARBOHYDRATE EPITOPE DEFINED BY AN ANTITUMOR MONOCLONAL-ANTIBODY DETECTED ON GLYCOPROTEINS AND A GLYCOLIPID BY IMMUNOBLOTTING / F. LEONI, S. MIOTTI, S. CANEVARI, S. SONNINO, M. RIPAMONTI, M. COLNAGHI. - In: HYBRIDOMA. - ISSN 0272-457X. - 5:4(1986), pp. 289-296.
|Titolo:||CARBOHYDRATE EPITOPE DEFINED BY AN ANTITUMOR MONOCLONAL-ANTIBODY DETECTED ON GLYCOPROTEINS AND A GLYCOLIPID BY IMMUNOBLOTTING|
|Settore Scientifico Disciplinare:||Settore BIO/10 - Biochimica|
|Data di pubblicazione:||1986|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1089/hyb.1986.5.289|
|Appare nelle tipologie:||01 - Articolo su periodico|