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EnglishAnti-GM2 IgM antibodies have been reported in some patients with dysimmune neuropathy or lower motor neuron syndrome. To determine whether these antibodies can induce complement-dependent cytolysis we performed a cytotoxicity assay on neuroblastoma cells with sera from seven patients with demyelinating dysimmune neuropathies and high titers of anti-GM2 IgM. As controls we used sera from seven patients with other anti-neural reactivities, six with the same neuropathies but no anti-GM2 or other anti-neural reactivity and from eight normal subjects. Of the seven positive sera tested, six induced complement-mediated cytotoxicity, while none of the controls had any relevant effect on neuroblastoma cells. Preincubation of positive sera with purified GM2 removed cytotoxic activity. Affinity purified anti-GM2 IgM had the same cytotoxic anti-GM2 effect of whole serum while serum or complement alone did not have any effect. In four anti-GM2-positive patients the percentage of cell lysis correlated with anti-GM2 titers and with IgM staining of neuroblastoma cells while in two the cytotoxic effect was higher than expected from antibody titers. Complement-mediated cell lysis induced by anti-GM2 IgM antibodies may be a possible mechanism of neural damage in patients with dysimmune neuropathy and high titers of anti-GM2 IgM antibodies.
Anti-GM(2) IgM antibody-induced complement-mediated cytotoxicity in patients with dysimmune neuropathies / B. Cavanna, H. Jiang, S. Allaria, M. Carpo, G. Scarlato, E. Nobile-Orazio. - In: JOURNAL OF NEUROIMMUNOLOGY. - ISSN 0165-5728. - 114:1-2(2001 Mar 01), pp. 226-31-231.
| Titolo: | Anti-GM(2) IgM antibody-induced complement-mediated cytotoxicity in patients with dysimmune neuropathies | |
| Autori: | SCARLATO, GUGLIELMO (Penultimo) NOBILE-ORAZIO, EDUARDO (Ultimo) | |
| Parole Chiave: | Antibodies; Cell culture; Complement; Cytotoxicity; Gangliosides; GM2; Neuropathy | |
| Settore Scientifico Disciplinare: | Settore MED/26 - Neurologia | |
| Data di pubblicazione: | 1-mar-2001 | |
| Rivista: | ||
| Tipologia: | Article (author) | |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/S0165-5728(00)00461-6 | |
| Appare nelle tipologie: | 01 - Articolo su periodico |
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