Anti-GM2 IgM antibodies have been reported in some patients with dysimmune neuropathy or lower motor neuron syndrome. To determine whether these antibodies can induce complement-dependent cytolysis we performed a cytotoxicity assay on neuroblastoma cells with sera from seven patients with demyelinating dysimmune neuropathies and high titers of anti-GM2 IgM. As controls we used sera from seven patients with other anti-neural reactivities, six with the same neuropathies but no anti-GM2 or other anti-neural reactivity and from eight normal subjects. Of the seven positive sera tested, six induced complement-mediated cytotoxicity, while none of the controls had any relevant effect on neuroblastoma cells. Preincubation of positive sera with purified GM2 removed cytotoxic activity. Affinity purified anti-GM2 IgM had the same cytotoxic anti-GM2 effect of whole serum while serum or complement alone did not have any effect. In four anti-GM2-positive patients the percentage of cell lysis correlated with anti-GM2 titers and with IgM staining of neuroblastoma cells while in two the cytotoxic effect was higher than expected from antibody titers. Complement-mediated cell lysis induced by anti-GM2 IgM antibodies may be a possible mechanism of neural damage in patients with dysimmune neuropathy and high titers of anti-GM2 IgM antibodies.
Anti-GM(2) IgM antibody-induced complement-mediated cytotoxicity in patients with dysimmune neuropathies / B. Cavanna, H. Jiang, S. Allaria, M. Carpo, G. Scarlato, E. Nobile-Orazio. - In: JOURNAL OF NEUROIMMUNOLOGY. - ISSN 0165-5728. - 114:1-2(2001 Mar 01), pp. 226-31-231.
|Titolo:||Anti-GM(2) IgM antibody-induced complement-mediated cytotoxicity in patients with dysimmune neuropathies|
SCARLATO, GUGLIELMO (Penultimo)
NOBILE-ORAZIO, EDUARDO (Ultimo)
|Parole Chiave:||Antibodies; Cell culture; Complement; Cytotoxicity; Gangliosides; GM2; Neuropathy|
|Settore Scientifico Disciplinare:||Settore MED/26 - Neurologia|
|Data di pubblicazione:||1-mar-2001|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/S0165-5728(00)00461-6|
|Appare nelle tipologie:||01 - Articolo su periodico|