A new analytical and semi-preparative high-performance liquid chromatographic method for the separation of a brain ganglioside mixture into individual components is described. Gangliosides were applied to a LiChrosorb-NH2 column and eluted with the solvent system acetonitrile-phosphate buffer at different volume ratios and ionic strengths. The elution profile was monitored by flow-through the detection of UV absorbance at 215 nm. The separation of mono- to polysialogangliosides was performed in one step in a total elution time lower than 90 min and with high reproducibility.

Normal-phase high-performance liquid chromatographic separation of non-derivatized ganglioside mixtures / G. Gazzotti, S. Sonnino, R. Ghidoni. - In: JOURNAL OF CHROMATOGRAPHY A. - ISSN 0021-9673. - 348:2(1985), pp. 371-378.

Normal-phase high-performance liquid chromatographic separation of non-derivatized ganglioside mixtures

S. Sonnino
Secondo
;
R. Ghidoni
Ultimo
1985

Abstract

A new analytical and semi-preparative high-performance liquid chromatographic method for the separation of a brain ganglioside mixture into individual components is described. Gangliosides were applied to a LiChrosorb-NH2 column and eluted with the solvent system acetonitrile-phosphate buffer at different volume ratios and ionic strengths. The elution profile was monitored by flow-through the detection of UV absorbance at 215 nm. The separation of mono- to polysialogangliosides was performed in one step in a total elution time lower than 90 min and with high reproducibility.
Settore BIO/10 - Biochimica
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/183833
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