A new analytical and semi-preparative high-performance liquid chromatographic method for the separation of a brain ganglioside mixture into individual components is described. Gangliosides were applied to a LiChrosorb-NH2 column and eluted with the solvent system acetonitrile-phosphate buffer at different volume ratios and ionic strengths. The elution profile was monitored by flow-through the detection of UV absorbance at 215 nm. The separation of mono- to polysialogangliosides was performed in one step in a total elution time lower than 90 min and with high reproducibility.
Normal-phase high-performance liquid chromatographic separation of non-derivatized ganglioside mixtures / G. Gazzotti, S. Sonnino, R. Ghidoni. - In: JOURNAL OF CHROMATOGRAPHY A. - ISSN 0021-9673. - 348:2(1985), pp. 371-378.
|Titolo:||Normal-phase high-performance liquid chromatographic separation of non-derivatized ganglioside mixtures|
SONNINO, SANDRO (Secondo)
GHIDONI, RICCARDO (Ultimo)
|Settore Scientifico Disciplinare:||Settore BIO/10 - Biochimica|
|Data di pubblicazione:||1985|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/S0021-9673(01)92475-6|
|Appare nelle tipologie:||01 - Articolo su periodico|