A study was made of the production of prostacyclin (PGI2) by cultured endothelial cells from the umbilical veins of mothers who smoked and of matched mothers who did not smoke. Cells were obtained from 58 umbilical cords from 22 apparently healthy women, 11 mild smokers (less than 15 cigarettes per day), and 25 heavy smokers (more than 15 cigarettes per day). Production of PGI2 by the cells was calculated by measuring the amount of 6-keto-prostaglandin F1 alpha released in the culture medium after the addition of arachidonic acid, by means of a specific radioimmunoassay. Endotheliai cells from mild and heavy smokers were significantly less able to grow and to reach confluency than cells from nonsmokers. Smoking also resulted in a marked reduction in the capacity of cultured cells to produce PGI2. This was particularly apparent for the cells from heavy smokers. Smoking during pregnancy appears to induce some modifications in the enzymes of the prostacyclin pathway which persist in endothelial cells undergoing replication in primary culture even in the absence of the pathogenic factor.

Maternal smoking and prostacyclin production by cultured endothelial cells from umbilical arteries / M. Busacca, G. Balconi, A. Pietra, M. Vergara-Dauden, G. de Gaetano, E. Dejana. - In: AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY. - ISSN 0002-9378. - 148:8(1984 Apr 15), pp. 1127-30-1130. [10.1016/0002-9378(84)90640-9]

Maternal smoking and prostacyclin production by cultured endothelial cells from umbilical arteries

M. Busacca
Primo
;
E. Dejana
Ultimo
1984

Abstract

A study was made of the production of prostacyclin (PGI2) by cultured endothelial cells from the umbilical veins of mothers who smoked and of matched mothers who did not smoke. Cells were obtained from 58 umbilical cords from 22 apparently healthy women, 11 mild smokers (less than 15 cigarettes per day), and 25 heavy smokers (more than 15 cigarettes per day). Production of PGI2 by the cells was calculated by measuring the amount of 6-keto-prostaglandin F1 alpha released in the culture medium after the addition of arachidonic acid, by means of a specific radioimmunoassay. Endotheliai cells from mild and heavy smokers were significantly less able to grow and to reach confluency than cells from nonsmokers. Smoking also resulted in a marked reduction in the capacity of cultured cells to produce PGI2. This was particularly apparent for the cells from heavy smokers. Smoking during pregnancy appears to induce some modifications in the enzymes of the prostacyclin pathway which persist in endothelial cells undergoing replication in primary culture even in the absence of the pathogenic factor.
Laboratory Examinations And Diagnoses; Research Methodology; Humans; Smoking--side effects; Studies; Pregnancy; Laboratory Procedures; Smoking; Growth; Histology; Behavior; Cells, Cultured; Umbilical Arteries; Endothelium; Adult; Epoprostenol; Examinations And Diagnoses; Male; Female; Comparative Studies; Biology
Settore MED/40 - Ginecologia e Ostetricia
Article (author)
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/183743
Citazioni
  • ???jsp.display-item.citation.pmc??? 0
  • Scopus 34
  • ???jsp.display-item.citation.isi??? ND
social impact