Cytoskeleton alterations of NIH/3T3 fibroblast monolayers transfected with Ha-ras-activated oncogene were studied by immunofluorescence, immunoelectron microscopy, and immunoelectrophoretic analysis of actin isoforms. Transformation foci were found to consist of cells with a round shape and rare stress fibers that spread sparsely, forming rare focal contacts and fibronexuses. The loss of stress fibers in transformed cells was confirmed by staining with rhodamine-phalloidin and with a fluorescinated anti-non-muscle cell actin antibody. The transformed cells were anchored to the substrate prominently by filaments that contained fibronectin, as showed by immunoelectron microscopy. A down-regulation of alpha-actin isoform was observed by immunofluorescence and immunoblotting analysis using a specific monoclonal antibody. The diffuse distribution of alpha-actin, lacking a specific association with stress fibers, challenges the hypothesis of a connection between alpha-actin down-regulation and stress fiber loss.
|Titolo:||Ultrastructural cytoskeleton alterations and modification of actin expression in the NIH/3T3 cell line after transformation with Ha-ras-activated oncogene|
MODINA, SILVIA CLOTILDE (Ultimo)
|Parole Chiave:||Animals; Proto-Oncogene Proteins p21(ras) ; Gene Expression ; Mice ; Fibroblasts ; Cytoskeleton ; Microscopy, Fluorescence ; Proto-Oncogene Proteins ; Actins ; Rhodamines ; Cell Line, Transformed ; Staining and Labeling ; Fluorescent Antibody Technique ; Cell Adhesion|
|Settore Scientifico Disciplinare:||Settore VET/01 - Anatomia degli Animali Domestici|
|Data di pubblicazione:||1990|
|Digital Object Identifier (DOI):||10.1002/cm.970150405|
|Appare nelle tipologie:||01 - Articolo su periodico|