2-Amino-1,5-dihydro-1-[methyl-H-2(3)] 4H-imidazol-4-one ([methyl-H-2(3)] creatinine) was obtained by acidification and heating (120-degrees-C, 120 min) of the [methyl -H-2(3)] N-amidinosarcosine (creatine) synthesised by methylation of guanidoacetate (GA) with S-adenosyl-[methyl-H-2(3)) L-methionine ([methyl-H-2(3)] AdoMet) in the presence of rat liver guanidoacetate methyltransferase (GAA-MT). Isotopic enrichment was 94.7 %.

ENZYMATIC-SYNTHESIS OF [METHYL-(H-2)3] CREATININE / C. ARCELLONI, R. PARONI, P.A.BONINI, F. MAGNI, M.G. KIENLE. - In: JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS. - ISSN 0362-4803. - 31:7(1992 Jul), pp. 505-517.

ENZYMATIC-SYNTHESIS OF [METHYL-(H-2)3] CREATININE

R. PARONI
Secondo
;
1992-07

Abstract

2-Amino-1,5-dihydro-1-[methyl-H-2(3)] 4H-imidazol-4-one ([methyl-H-2(3)] creatinine) was obtained by acidification and heating (120-degrees-C, 120 min) of the [methyl -H-2(3)] N-amidinosarcosine (creatine) synthesised by methylation of guanidoacetate (GA) with S-adenosyl-[methyl-H-2(3)) L-methionine ([methyl-H-2(3)] AdoMet) in the presence of rat liver guanidoacetate methyltransferase (GAA-MT). Isotopic enrichment was 94.7 %.
[METHYL-2H3] CREATININE; GUANIDOACETATE METHYLTRANSFERASE; S-ADENOSYL-[METHYL-2H3] L-METHIONINE; ISOTOPE DILUTION MASS SPECTROMETRY; SERUM; PLASMA; URINE
Settore BIO/10 - Biochimica
Settore BIO/12 - Biochimica Clinica e Biologia Molecolare Clinica
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/183538
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