We report a rather simple method to determine glucose concentration in serum, using isotope dilution mass spectrometry and [C-13(6)]glucose as internal standard. The procedure involves a single step of sample purification and the conversion of the analyte into its aldononitrile pentaacetate. The between-day and within-day contribution to total variance for a single measurement was determined by assaying Standard Reference Material (SRM) 909 serum. The method was then applied to measurement of glucose concentration in three lyophilized sera: SRM 909 and two other commercially available sera. In the two studies, the concentration of SRM 909 serum was found to be 0.8% above and 0.3% below the reported value (6.25 mmol/L), respectively; the overall coefficient of variation for determinations in all sera ranged from 0.37% to 0.56%. The precision and the accuracy of the method satisfy the requirements for a Definitive Method.

DETERMINATION OF SERUM GLUCOSE BY ISOTOPE-DILUTION MASS-SPECTROMETRY - CANDIDATE DEFINITIVE METHOD / F. MAGNI, R. PARONI, P.A. BONINI, M.G. KIENLE. - In: CLINICAL CHEMISTRY. - ISSN 0009-9147. - 38:3(1992 Mar), pp. 381-385.

DETERMINATION OF SERUM GLUCOSE BY ISOTOPE-DILUTION MASS-SPECTROMETRY - CANDIDATE DEFINITIVE METHOD

R. PARONI
Secondo
;
1992-03

Abstract

We report a rather simple method to determine glucose concentration in serum, using isotope dilution mass spectrometry and [C-13(6)]glucose as internal standard. The procedure involves a single step of sample purification and the conversion of the analyte into its aldononitrile pentaacetate. The between-day and within-day contribution to total variance for a single measurement was determined by assaying Standard Reference Material (SRM) 909 serum. The method was then applied to measurement of glucose concentration in three lyophilized sera: SRM 909 and two other commercially available sera. In the two studies, the concentration of SRM 909 serum was found to be 0.8% above and 0.3% below the reported value (6.25 mmol/L), respectively; the overall coefficient of variation for determinations in all sera ranged from 0.37% to 0.56%. The precision and the accuracy of the method satisfy the requirements for a Definitive Method.
DEFINITIVE METHODS; GLUCOSE; ID-MS; STANDARD REFERENCE MATERIAL
Settore BIO/10 - Biochimica
Settore BIO/12 - Biochimica Clinica e Biologia Molecolare Clinica
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/183537
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