A radioimmunoassay (RIA) for measuring isotype-specific antibodies to the myelin-associated glycoprotein (MAG) was developed using radiolabeled CNS MAG in a double-antibody precipitation system. Anti-MAG activity was detected by RIA only in patients with neuropathy and anti-MAG M proteins. Anti-MAG IgM or IgG antibodies were not detected in serum of patients with Guillain-Barré syndrome, chronic relapsing polyneuritis, or multiple sclerosis (MS). Some patients with anti-MAG IgM M proteins also had complexes of IgG or IgA bound to the M protein. In one patient, anti-CNS MAG activity was detected by RIA, but not by ELISA or immunoblot. Anti-MAG antibody activity in patients with neuropathy seems to be isotypically restricted, and there is no evidence for antibody reactivity to MAG in other demyelinating diseases.
|Titolo:||Anti-MAG antibody and antibody complexes: detection by radioimmunoassay|
NOBILE-ORAZIO, EDUARDO (Primo)
|Parole Chiave:||Immunoglobulin A; Myelin-Associated Glycoprotein; Myelin Proteins; Peripheral Nervous System Diseases; Humans; Immunoglobulin G; Central Nervous System Diseases; Immunosorbent Techniques; Radioimmunoassay; Immunoglobulin M; Antibodies, Anti-Idiotypic|
|Settore Scientifico Disciplinare:||Settore MED/26 - Neurologia|
|Data di pubblicazione:||lug-1985|
|Appare nelle tipologie:||01 - Articolo su periodico|