Low density lipoprotein (LDL) cholesterol is known to be oxidized both in vitro and in vivo giving rise to oxygenated sterols. Conflicting results, however, have been reported concerning both the nature and the relative concentrations of these compounds in oxidized human LDL. We examined the extracts obtained from Cu2+-oxidized LDL. Thin layer chromatography analysis showed that the sterol mixture became more complex with reaction time. Analysis of the components by thin layer chromatography and mass spectrometry allowed to establish that 7alpha- and 7beta-hydroperoxycholest-5-en-3beta-ol (7alphaOOH and betaOOH) are largely prevalent among the oxysterols at early times of oxidation. These hydroperoxy derivatives have not been previously identified in oxidized LDL. The concentration of 7-hydroperoxycholest-5-en-3beta-ol decreased with oxidation time with a concomitant increase of cholest-5-en-3beta,7alpha-diol (7alphaOH), cholest-5-en-3beta,7beta-diol (7betaOH), cholesta-3,5-dien-7-one (CD) and cholest-5-en-3beta-ol-7-one (7CO). After 24 h of oxidation a minor component of the LDL sterols was cholestan-3beta-ol-5,6-oxide (EP).
|Titolo:||EVIDENCE FOR THE PRESENCE OF 7-HYDROPEROXYCHOLEST-5-EN-3-BETA-OL IN OXIDIZED HUMAN LDL|
CATAPANO, ALBERICO LUIGI (Penultimo)
|Parole Chiave:||OXYSTEROLS ; CHOLESTEROL HYDROPEROXIDES ; CHOLESTEROL OXIDATION ; OXIDIZED LDL|
|Settore Scientifico Disciplinare:||Settore BIO/10 - Biochimica|
|Data di pubblicazione:||1992|
|Digital Object Identifier (DOI):||10.1016/0009-3084(92)90057-V|
|Appare nelle tipologie:||01 - Articolo su periodico|