Low density lipoprotein (LDL) cholesterol is known to be oxidized both in vitro and in vivo giving rise to oxygenated sterols. Conflicting results, however, have been reported concerning both the nature and the relative concentrations of these compounds in oxidized human LDL. We examined the extracts obtained from Cu2+-oxidized LDL. Thin layer chromatography analysis showed that the sterol mixture became more complex with reaction time. Analysis of the components by thin layer chromatography and mass spectrometry allowed to establish that 7alpha- and 7beta-hydroperoxycholest-5-en-3beta-ol (7alphaOOH and betaOOH) are largely prevalent among the oxysterols at early times of oxidation. These hydroperoxy derivatives have not been previously identified in oxidized LDL. The concentration of 7-hydroperoxycholest-5-en-3beta-ol decreased with oxidation time with a concomitant increase of cholest-5-en-3beta,7alpha-diol (7alphaOH), cholest-5-en-3beta,7beta-diol (7betaOH), cholesta-3,5-dien-7-one (CD) and cholest-5-en-3beta-ol-7-one (7CO). After 24 h of oxidation a minor component of the LDL sterols was cholestan-3beta-ol-5,6-oxide (EP).
EVIDENCE FOR THE PRESENCE OF 7-HYDROPEROXYCHOLEST-5-EN-3-BETA-OL IN OXIDIZED HUMAN LDL / B. MALAVASI, M. RASETTI, P. ROMA, R. FOGLIATTO, P. ALLEVI, A. CATAPANO, G. GALLI. - In: CHEMISTRY AND PHYSICS OF LIPIDS. - ISSN 0009-3084. - 62:3(1992), pp. 209-214.
EVIDENCE FOR THE PRESENCE OF 7-HYDROPEROXYCHOLEST-5-EN-3-BETA-OL IN OXIDIZED HUMAN LDL
P. ALLEVI;A. CATAPANOPenultimo
;
1992
Abstract
Low density lipoprotein (LDL) cholesterol is known to be oxidized both in vitro and in vivo giving rise to oxygenated sterols. Conflicting results, however, have been reported concerning both the nature and the relative concentrations of these compounds in oxidized human LDL. We examined the extracts obtained from Cu2+-oxidized LDL. Thin layer chromatography analysis showed that the sterol mixture became more complex with reaction time. Analysis of the components by thin layer chromatography and mass spectrometry allowed to establish that 7alpha- and 7beta-hydroperoxycholest-5-en-3beta-ol (7alphaOOH and betaOOH) are largely prevalent among the oxysterols at early times of oxidation. These hydroperoxy derivatives have not been previously identified in oxidized LDL. The concentration of 7-hydroperoxycholest-5-en-3beta-ol decreased with oxidation time with a concomitant increase of cholest-5-en-3beta,7alpha-diol (7alphaOH), cholest-5-en-3beta,7beta-diol (7betaOH), cholesta-3,5-dien-7-one (CD) and cholest-5-en-3beta-ol-7-one (7CO). After 24 h of oxidation a minor component of the LDL sterols was cholestan-3beta-ol-5,6-oxide (EP).Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.