A sensitive and specific procedure is described for the determination of therapeutically relevant concentrations of L-dopa in plasma and plasma ultrafiltrates (free fraction) by high performance liquid chromatography with electrochemical detection. In plasma samples from healthy adult subjects (n = 15) spiked with L-dopa (500 μg l -1) the free fraction averaged 76 ± 8% (range 61-84%). Free fraction values increased by 38% with increasing plasma concentrations of L-dopa from 100-5000 μg l -1. L-dopa free fraction was not affected by the presence of 3-o-methyl-dopa a concentrations up to 10 000 μg l.
Concentrations of L-dopa in plasma and plasma ultrafiltrates / Rizzo V, Memmi M, Moratti R, Melzi d'Eril G, Perucca. - In: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS. - ISSN 0731-7085. - 14:8-10(1996 Jun), pp. 1043-1046. [10.1016/S0731-7085(96)01753-0]
Concentrations of L-dopa in plasma and plasma ultrafiltrates.
Melzi d'Eril G;
1996-06
Abstract
A sensitive and specific procedure is described for the determination of therapeutically relevant concentrations of L-dopa in plasma and plasma ultrafiltrates (free fraction) by high performance liquid chromatography with electrochemical detection. In plasma samples from healthy adult subjects (n = 15) spiked with L-dopa (500 μg l -1) the free fraction averaged 76 ± 8% (range 61-84%). Free fraction values increased by 38% with increasing plasma concentrations of L-dopa from 100-5000 μg l -1. L-dopa free fraction was not affected by the presence of 3-o-methyl-dopa a concentrations up to 10 000 μg l.
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