In this study oxidation of low-density lipoprotein (LDL) induced by different Cu2+ concentrations was investigated. Lipid peroxidation was assessed by monitoring low-level chemiluminescence (LL-CL), conjugated diene hydroperoxide (CD) and α-tocopherol (TocOH), the major lipophilic antioxidant in LDL. At high Cu2+ concentration, LDL oxidation was characterised by CD formation, LL-CL emission and TocOH consumption. At low Cu2+ concentration, CD formation was independent of LL-CL and occurred in the presence of TocOH. Thus, two different mechanisms lead to lipid peroxide formation in LDL. The combination of CD assay and LL-CL monitoring makes it possible to distinguish the autocatalytic mechanism of CD formation and that associated with TocOH, found at a high and a low rate of initiation, respectively. Copyright (C) 1999 Federation of European Biochemical Societies.
Mechanistic aspects of the relationship between low-level chemiluminescence and lipid peroxides in oxidation of low-density lipoprotein. / Albertini R, De Luca G, Palladini G, Passi A, Melzi d'Eril GV, Abuja PM.. - In: FEBS LETTERS. - ISSN 0014-5793. - 459:1(1999 Oct), pp. 47-50.
Titolo: | Mechanistic aspects of the relationship between low-level chemiluminescence and lipid peroxides in oxidation of low-density lipoprotein. | |
Autori: | ||
Settore Scientifico Disciplinare: | Settore BIO/12 - Biochimica Clinica e Biologia Molecolare Clinica | |
Data di pubblicazione: | ott-1999 | |
Rivista: | ||
Tipologia: | Article (author) | |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/S0014-5793(99)01217-X | |
Appare nelle tipologie: | 01 - Articolo su periodico |