The performance of an enzymatic colorimetric method for the determination of inorganic phosphorus in serum and urine is described. Phosphate ions react with inosine in the presence of purine nucleoside phosphorylase to form hypoxanthine; this is oxidized by xanthine oxidase to uric acid with production of hydrogen peroxide. The latter is determined with the aid of the chromogen system peroxidase/4-aminophenazone/N-ethyl-N-(3-methylphenyl)-N'-acetyl- ethylenediamine, the coloured product being measured at 555 nm. This series of reactions is completed in 5 min at 37°C. The test is linear up to 240 mg/l. Analytical recovery in serum averaged 101.2 ± 1.2% and in urine 101.9 ± 3.2%. Within-run and between-run precision studies in serum and urine samples gave CVs ≤ 4.54% (at 22.0 mg/l). Results obtained by this method agree (r = ≥ 0.983) with the molybdate UV and molybdenum blue methods. Interference by endogenous substances, including organic phosphate, was negligible.

Enzymatic colorimetric method for the determination of inorganic phosphorus in serum and urine / G. Berti, P. Fossati, G. Tarenghi, C. Musitelli, G. Melzi d'Eril. - In: CLINICAL CHEMISTRY AND LABORATORY MEDICINE. - ISSN 1437-4331. - 26:6(1988), pp. 399-404.

Enzymatic colorimetric method for the determination of inorganic phosphorus in serum and urine.

G. Melzi d'Eril
1988

Abstract

The performance of an enzymatic colorimetric method for the determination of inorganic phosphorus in serum and urine is described. Phosphate ions react with inosine in the presence of purine nucleoside phosphorylase to form hypoxanthine; this is oxidized by xanthine oxidase to uric acid with production of hydrogen peroxide. The latter is determined with the aid of the chromogen system peroxidase/4-aminophenazone/N-ethyl-N-(3-methylphenyl)-N'-acetyl- ethylenediamine, the coloured product being measured at 555 nm. This series of reactions is completed in 5 min at 37°C. The test is linear up to 240 mg/l. Analytical recovery in serum averaged 101.2 ± 1.2% and in urine 101.9 ± 3.2%. Within-run and between-run precision studies in serum and urine samples gave CVs ≤ 4.54% (at 22.0 mg/l). Results obtained by this method agree (r = ≥ 0.983) with the molybdate UV and molybdenum blue methods. Interference by endogenous substances, including organic phosphate, was negligible.
Settore BIO/12 - Biochimica Clinica e Biologia Molecolare Clinica
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/183014
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