The present data show that the gene expression of FGF-1 and FGF-2 is regulated by corticosteroids in rat type 1 astrocytes. In particular, the gene expression of FGF-1 is modulated by corticosteroids acting both on type I (minerocorticoid) and type II (glucocorticoid) receptors. In fact, at short times of exposure (2 h) a slight decrease in FGF-1 mRNA levels is induced by deoxycorticosterone, a steroid able to interact with the type I receptors; a similar effect is observed at 6 h following exposure to corticosterone or its 5alpha-reduced metabolite, dihydrocorticosterone. Conversely, at longer times of exposure (24 h) corticosterone is able to strongly increase FGF-1 mRNA levels. Both effects of corticosterone (inhibition and stimulation) were duplicated by dexamethasone, indicating that both effects occur via the type II receptors. Interestingly, the 5alpha-3alpha-reduced metabolite of deoxycorticosterone, tetrahydrodeoxycorticosterone, which does not interact with either corticosteroid receptors, is able to stimulate (at 6 and 24 h of exposure) the gene expression of FGF-1. It is possible that this effect might be induced via the GABA(A) receptor, since muscimol, an agonist of this receptor, exerts a similar effect. The situation is different in the case of FGF-2. The mRNA levels of this growth factor are only stimulated by steroids interacting with type II receptors. Altogether, these observations indicate that corticosteroids modulate the levels of FGF-1 and FGF-2 gene expression in astroglial cells by interaction with classical (type I and II) or nonclassical (GABA(A) receptor) steroid receptors.
Corticosteroids regulate the gene expression of FGF-1 and FGF-2 in cultured rat astrocytes / V. Magnaghi, M.A. Riva, I. Cavarretta, L. Martini, R.C. Melcangi. - In: JOURNAL OF MOLECULAR NEUROSCIENCE. - ISSN 0895-8696. - 15:1(2000 Aug), pp. 11-18.
|Titolo:||Corticosteroids regulate the gene expression of FGF-1 and FGF-2 in cultured rat astrocytes|
MAGNAGHI, VALERIO (Primo)
RIVA, MARCO ANDREA (Secondo)
MARTINI, LUCIANO (Penultimo)
MELCANGI, COSIMO (Ultimo)
|Parole Chiave:||5α- and 5α-3α-reduced metabolites; Astrocytes; Corticosterone; FGF-1; FGF-2; GABAA receptor; Type I and II corticosteroid receptors|
|Settore Scientifico Disciplinare:||Settore MED/13 - Endocrinologia|
Settore BIO/14 - Farmacologia
Settore BIO/09 - Fisiologia
|Data di pubblicazione:||ago-2000|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1385/JMN:15:1:11|
|Appare nelle tipologie:||01 - Articolo su periodico|