UK114 is a tumor antigen expressed by various malignant neoplasms, Tile complete amino acid sequence of UK114 purified from goat liver has been determined by automated Edman degradation of CNBr and endoproteinase Lys-C peptides. The protein contains 137 amino acid residues, which corresponds to a molecular mass of 14229 Da. MALDI-TOF analysis resulted in a molecular weight of 14290, suggesting that the N-terminal Met residue is acetylated, Sequence comparison shows that UK114 from goat liver (1) has 77% identity with a previously described 23 kDa protein from rat liver (Levy-Favatier et al. (1993) Eur. J. Biochem. 212, 665-673), (2) shares a very high degree of similarity with a family of prokaryotic and eukaryotic hypothetic proteins whose function have not yet been characterized, and (3) exhibits a significant similarity to a group of tumor-associated antigens which belongs to a superfamily of heat shock proteins, acting as possible targets for the host's antitumor immunity.

The primary structure of UK114 tumor antigen / F. Ceciliani, L. Faotto, A. Negri, I. Colombo, B. Berra, A. Baertorelli, S. Ronchi. - In: FEBS LETTERS. - ISSN 0014-5793. - 393:2-3(1996), pp. 147-150.

The primary structure of UK114 tumor antigen

F. Ceciliani
Primo
;
A. Negri;I. Colombo;B. Berra;S. Ronchi
Ultimo
1996

Abstract

UK114 is a tumor antigen expressed by various malignant neoplasms, Tile complete amino acid sequence of UK114 purified from goat liver has been determined by automated Edman degradation of CNBr and endoproteinase Lys-C peptides. The protein contains 137 amino acid residues, which corresponds to a molecular mass of 14229 Da. MALDI-TOF analysis resulted in a molecular weight of 14290, suggesting that the N-terminal Met residue is acetylated, Sequence comparison shows that UK114 from goat liver (1) has 77% identity with a previously described 23 kDa protein from rat liver (Levy-Favatier et al. (1993) Eur. J. Biochem. 212, 665-673), (2) shares a very high degree of similarity with a family of prokaryotic and eukaryotic hypothetic proteins whose function have not yet been characterized, and (3) exhibits a significant similarity to a group of tumor-associated antigens which belongs to a superfamily of heat shock proteins, acting as possible targets for the host's antitumor immunity.
Amino acid sequence; Tumor antigen; UK114
Settore BIO/10 - Biochimica
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/182606
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