A rise of cytosolic Ca2+ is induced by NGF in rat pheochromocytoma PC12 and bovine chromaffin cells investigated (both in suspension and while attached to polyornithine-coated glass slides) by fluorescence techniques (with quin-2 and fura-2). The effect of NGF on [Ca2+]i is delayed (30-40 s of lag phase), slow (t1/2 = 40 s), relatively small (+50-75%) and persistent (over 10 min). It is due to Ca2+ influx (requires extracellular Ca2+ greater than 10 microM) through a pathway different from the voltage-gated Ca2+ channel, possibly accompanied by intracellular Ca2+ redistribution, and might play a messenger role in NGF action.
|Titolo:||Early rise of cytosolic Ca2+ induced by NGF in PC12 and chromaffin cells|
VICENTINI, LUCIA (Penultimo)
|Parole Chiave:||Nerve growth factor Ca2+ Second messenger Phosphatidylinositol Quin-2 Fura-2|
|Settore Scientifico Disciplinare:||Settore BIO/14 - Farmacologia|
|Data di pubblicazione:||10-nov-1986|
|Digital Object Identifier (DOI):||10.1016/0014-5793(86)81529-0|
|Appare nelle tipologie:||01 - Articolo su periodico|