A rise of cytosolic Ca2+ is induced by NGF in rat pheochromocytoma PC12 and bovine chromaffin cells investigated (both in suspension and while attached to polyornithine-coated glass slides) by fluorescence techniques (with quin-2 and fura-2). The effect of NGF on [Ca2+]i is delayed (30-40 s of lag phase), slow (t1/2 = 40 s), relatively small (+50-75%) and persistent (over 10 min). It is due to Ca2+ influx (requires extracellular Ca2+ greater than 10 microM) through a pathway different from the voltage-gated Ca2+ channel, possibly accompanied by intracellular Ca2+ redistribution, and might play a messenger role in NGF action.
Early rise of cytosolic Ca2+ induced by NGF in PC12 and chromaffin cells / A. Pandiella-Alonso, A. Malgaroli, L. M. Vicentini, J. Meldolesi. - In: FEBS LETTERS. - ISSN 0014-5793. - 208:1(1986 Nov 10), pp. 48-51.
Titolo: | Early rise of cytosolic Ca2+ induced by NGF in PC12 and chromaffin cells | |
Autori: | VICENTINI, LUCIA (Penultimo) | |
Parole Chiave: | Nerve growth factor Ca2+ Second messenger Phosphatidylinositol Quin-2 Fura-2 | |
Settore Scientifico Disciplinare: | Settore BIO/14 - Farmacologia | |
Data di pubblicazione: | 10-nov-1986 | |
Rivista: | ||
Tipologia: | Article (author) | |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/0014-5793(86)81529-0 | |
Appare nelle tipologie: | 01 - Articolo su periodico |