The majority of B cell nonHodgkin's lymphomas (NHLs) are composed of a genotypically identical cell population characterized by a unique immunoglobulin (Ig) VDJ gene rearrangement which is customarily documented by Southern blot hybridization analysis of fresh tissue. Sometimes, however, this approach cannot be used because of an insufficient quantity of tissue or the unavailability of fresh tissue. Therefore, alternative strategies should be designed in order to overcome these limitations.

Detection of immunoglobulin gene rearrangement of B cell non-Hodgkin's lymphomas and leukemias in fresh, unfixed and formalin-fixed, paraffin-embedded tissue by polymerase chain reaction / G. Inghirami, M. J. Szabolcs, H. T. Yee, P. Corradini, E. Cesarman, D. M. Knowles. - In: LABORATORY INVESTIGATION. - ISSN 0023-6837. - 68:6(1993 Jun), pp. 746-57-757.

Detection of immunoglobulin gene rearrangement of B cell non-Hodgkin's lymphomas and leukemias in fresh, unfixed and formalin-fixed, paraffin-embedded tissue by polymerase chain reaction

P. Corradini;
1993-06

Abstract

The majority of B cell nonHodgkin's lymphomas (NHLs) are composed of a genotypically identical cell population characterized by a unique immunoglobulin (Ig) VDJ gene rearrangement which is customarily documented by Southern blot hybridization analysis of fresh tissue. Sometimes, however, this approach cannot be used because of an insufficient quantity of tissue or the unavailability of fresh tissue. Therefore, alternative strategies should be designed in order to overcome these limitations.
Sensitivity and Specificity; Paraffin Embedding; Humans; Gene Rearrangement, B-Lymphocyte, Heavy Chain; Genes, Immunoglobulin; Formaldehyde; Cloning, Molecular; DNA, Neoplasm; Lymphoma, B-Cell; Polymerase Chain Reaction; Base Sequence; Molecular Sequence Data; Immunoglobulin Variable Region; Leukemia, B-Cell; Tissue Fixation
Settore MED/15 - Malattie del Sangue
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/182255
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