We describe here conditions for the in vitro preparation of the main metabolite of cyclosporin A, namely AM1, with rabbit liver enzymes. The compound was purified and was identified by comparison with the metabolite isolated from urine of rats treated with [3H]CsA and by its Mass-Spectrometry Fast Ion Bombardment (MS-FIB) and IHNMR spectra. The procedure is simple and with the enzyme fraction derived from one rabbit liver 34 mg of the pure metabolite can be obtained.
EFFICIENT PRODUCTION OF THE CYCLOSPORINE METABOLITE AM1 BY RABBIT HEPATIC-ENZYMES / R. PARONI, I. FERMO, L. IAVARONE, L.M. DELPUPPO, L. DEANGELIS, M. KIENLE. - In: PHARMACOLOGICAL RESEARCH. - ISSN 1043-6618. - 28:1(1993 Jul), pp. 73-84.
EFFICIENT PRODUCTION OF THE CYCLOSPORINE METABOLITE AM1 BY RABBIT HEPATIC-ENZYMES
R. PARONIPrimo
;
1993
Abstract
We describe here conditions for the in vitro preparation of the main metabolite of cyclosporin A, namely AM1, with rabbit liver enzymes. The compound was purified and was identified by comparison with the metabolite isolated from urine of rats treated with [3H]CsA and by its Mass-Spectrometry Fast Ion Bombardment (MS-FIB) and IHNMR spectra. The procedure is simple and with the enzyme fraction derived from one rabbit liver 34 mg of the pure metabolite can be obtained.
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