The ability of endogenous glutathione (GSH) to modify the activity of the enzyme xanthine oxidase (XO) in rat liver was investigated. The effect of hepatic GSH depletion on the conversion of xanthine dehydrogenase (XDH) (EC 1.1.1.204) to XO (EC 1.1.3.22) was determined 10 min after i.p. administration of different amounts of diethylmaleate to fasted rats. After administration of 400 mg/kg, total hepatic non-protein GSH (reduced + oxidized GSH) decreased significantly to 14% of controls. In this condition the level of oxidized GSH was unchanged and no lipid peroxidation was observed, while a significant increase of reversible XO and a minor increase of the irreversible form of the enzyme was detected.
EFFECT OF GLUTATHIONE DEPLETION ON THE CONVERSION OF XANTHINE DEHYDROGENASE TO OXIDASE IN RAT-LIVER / G. CIGHETTI, S. DEBIASI, R. PARONI. - In: BIOCHEMICAL PHARMACOLOGY. - ISSN 0006-2952. - 45:11(1993 Jun 09), pp. 2359-2361. [10.1016/0006-2952(93)90213-G]
EFFECT OF GLUTATHIONE DEPLETION ON THE CONVERSION OF XANTHINE DEHYDROGENASE TO OXIDASE IN RAT-LIVER
G. CighettiPrimo
;R. ParoniUltimo
1993
Abstract
The ability of endogenous glutathione (GSH) to modify the activity of the enzyme xanthine oxidase (XO) in rat liver was investigated. The effect of hepatic GSH depletion on the conversion of xanthine dehydrogenase (XDH) (EC 1.1.1.204) to XO (EC 1.1.3.22) was determined 10 min after i.p. administration of different amounts of diethylmaleate to fasted rats. After administration of 400 mg/kg, total hepatic non-protein GSH (reduced + oxidized GSH) decreased significantly to 14% of controls. In this condition the level of oxidized GSH was unchanged and no lipid peroxidation was observed, while a significant increase of reversible XO and a minor increase of the irreversible form of the enzyme was detected.
Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
