In recombinase-deficient (RAG-2(-/-)) mice, double-negative thymocytes can be stimulated to proliferate and differentiate by anti-CD3 Abs. CD3 molecules are expressed on the surface of these cells in association with calnexin. In this study, we show that ζ-chains can be recovered as phosphorylated proteins in association with phosphorylated ZAP-70 from anti- CD3-stimulated RAG-2(-/-) thymocytes, even though they are not demonstrably associated with the CD3/calnexin complex. The lack of a physical association of ζ dimers with the CD3 complex in RAG-2(-/-) thymocytes and also in a pre- TCR-expressing cell line, as well as the efficient association of ζ dimers with ZAP-70 in the RAG-2(-/-) thymocytes, suggest that these ζ-chain dimers could contribute to pre-TCR signaling. This idea is supported by the finding that in RAG-2(-/-) ζ-deficient thymocytes, ZAP-70 and p120(cbl) were only weakly phosphorylated.

Surface expression and functional competence of CD3-independent TCR ζ-chains in immature thymocytes / F Grassi, E Barbier, S Porcellini, H von Boehmer, PA Cazenave. - In: JOURNAL OF IMMUNOLOGY. - ISSN 0022-1767. - 162:5(1999), pp. 2589-2596.

Surface expression and functional competence of CD3-independent TCR ζ-chains in immature thymocytes

F Grassi
Primo
;
1999

Abstract

In recombinase-deficient (RAG-2(-/-)) mice, double-negative thymocytes can be stimulated to proliferate and differentiate by anti-CD3 Abs. CD3 molecules are expressed on the surface of these cells in association with calnexin. In this study, we show that ζ-chains can be recovered as phosphorylated proteins in association with phosphorylated ZAP-70 from anti- CD3-stimulated RAG-2(-/-) thymocytes, even though they are not demonstrably associated with the CD3/calnexin complex. The lack of a physical association of ζ dimers with the CD3 complex in RAG-2(-/-) thymocytes and also in a pre- TCR-expressing cell line, as well as the efficient association of ζ dimers with ZAP-70 in the RAG-2(-/-) thymocytes, suggest that these ζ-chain dimers could contribute to pre-TCR signaling. This idea is supported by the finding that in RAG-2(-/-) ζ-deficient thymocytes, ZAP-70 and p120(cbl) were only weakly phosphorylated.
Settore BIO/13 - Biologia Applicata
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/181970
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