The mitogens serum, vasopressin and bradykinin stimulate a significant rise in the inositol phosphate content of cultured human fibroblasts within 10 seconds, while serum- and bradykinin-stimulated arachidonic acid release does not occur until after 30 seconds. The release of inositol phosphates is not secondary to a rise in Ca activity since the Ca ionophore ionomycin does not stimulate release of inositol phosphates. Moreover, we show that phospholipase C in human fibroblasts is activated by these mitogens at resting Ca levels since TMB-8, which blocks the mitogen-induced rise in Ca activity, does not affect the serum-stimulated accumulation of inositol phosphates.

Serum, bradykinin and vasopressin stimulate release of inositol phosphates from human fibroblasts / L. M. Vicentini, M. L. Villereal. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - 123:2(1984 Sep 17), pp. 663-670.

Serum, bradykinin and vasopressin stimulate release of inositol phosphates from human fibroblasts

L. M. Vicentini
Primo
;
1984-09-17

Abstract

The mitogens serum, vasopressin and bradykinin stimulate a significant rise in the inositol phosphate content of cultured human fibroblasts within 10 seconds, while serum- and bradykinin-stimulated arachidonic acid release does not occur until after 30 seconds. The release of inositol phosphates is not secondary to a rise in Ca activity since the Ca ionophore ionomycin does not stimulate release of inositol phosphates. Moreover, we show that phospholipase C in human fibroblasts is activated by these mitogens at resting Ca levels since TMB-8, which blocks the mitogen-induced rise in Ca activity, does not affect the serum-stimulated accumulation of inositol phosphates.
Calcium; Bradykinin; Humans; Fibroblasts; Arachidonic Acids; Blood; Ionomycin; Vasopressins; Ethers; Sugar Phosphates; Inositol Phosphates; Time Factors; Type C Phospholipases; Arachidonic Acid
Settore BIO/14 - Farmacologia
Article (author)
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/181905
Citazioni
  • ???jsp.display-item.citation.pmc??? 13
  • Scopus 56
  • ???jsp.display-item.citation.isi??? ND
social impact