This study examined the ability of immature cat oocytes to survive after cryopreservation by evaluating their subsequent development following maturation in vitro. The effect of slow and ultrarapid freezing using one of two cryoprotectants dimethylsulphoxide (DMSO) or 1,2-propanediol (PROH) at different concentrations (1.5 or 3.0 mol-1) and the slow freezing with the cryoprotectant ethylene glycol (EG 1.5 mol l-1 and 3.0 mol l-1) were tested. Morphology, resumption of meiosis and metaphase II rates of oocytes were recorded after thawing. Freshly collected oocytes were used as controls. Results indicate that immature cat oocytes can survive, resume meiosis and achieve metaphase II in vitro after freezing. The highest rates of resumption of meiosis and metaphase II were achieved with slow freezing and 1.5 mol DMSO or EG l-1 (DMSO: 47.4%, 18/38 and 23.7%, 9/38 and EG: 52%, 13/25 and 20%, 5/25, respectively). The ultrarapid procedure did not result in resumption of meiosis in vitro, despite intact morphology of the oocytes after thawing. These results suggest that morphology of oocytes after freezing and thawing has no predictive value for their ability to resume meiosis.

Effects of slow and ultrarapid freezing on morphology and resumption of meiosis in immature cat oocytes / G.C. Luvoni, P. Pellizzari, M. Battocchio. - In: JOURNAL OF REPRODUCTION AND FERTILITY. SUPPLEMENT. - ISSN 0449-3087. - 51(1997), pp. 93-98. ((Intervento presentato al 3. convegno International Symposium on Canine and Feline Reproduction tenutosi a Veldhoven nel 1996.

Effects of slow and ultrarapid freezing on morphology and resumption of meiosis in immature cat oocytes

G.C. Luvoni
Primo
;
M. Battocchio
Ultimo
1997

Abstract

This study examined the ability of immature cat oocytes to survive after cryopreservation by evaluating their subsequent development following maturation in vitro. The effect of slow and ultrarapid freezing using one of two cryoprotectants dimethylsulphoxide (DMSO) or 1,2-propanediol (PROH) at different concentrations (1.5 or 3.0 mol-1) and the slow freezing with the cryoprotectant ethylene glycol (EG 1.5 mol l-1 and 3.0 mol l-1) were tested. Morphology, resumption of meiosis and metaphase II rates of oocytes were recorded after thawing. Freshly collected oocytes were used as controls. Results indicate that immature cat oocytes can survive, resume meiosis and achieve metaphase II in vitro after freezing. The highest rates of resumption of meiosis and metaphase II were achieved with slow freezing and 1.5 mol DMSO or EG l-1 (DMSO: 47.4%, 18/38 and 23.7%, 9/38 and EG: 52%, 13/25 and 20%, 5/25, respectively). The ultrarapid procedure did not result in resumption of meiosis in vitro, despite intact morphology of the oocytes after thawing. These results suggest that morphology of oocytes after freezing and thawing has no predictive value for their ability to resume meiosis.
Cat ; oocytes ; freezing
Settore VET/10 - Clinica Ostetrica e Ginecologia Veterinaria
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/181668
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