An early event during the induction of skin sensitization is the production by keratinocytes (kc) of cytokines. Considering the role of kc in skin inflammatory and immune responses, we investigated the effect of three skin allergens (oxazolone, nickel sulfate and mixture of methylisothiazolinone and methylcholoisotiazolone) on IL-1α production by a murine kc cell line (HEL30). Confluent cells were treated for different times with various concentrations of oxazolone, NiSO4 and mixture of methylisothiazolinone and methylcholoisotiazolone. IL-1α was measured by ELISA both in conditioned media and cell-associated. All chemicals were able to induce a dose-response production of IL-1α. This increase was related to an increase in protein synthesis as measured by 3H-leucine incorporation. At high concentrations all chemicals were cytotoxic as measured by LDH release. In the same cell system we also measured the effect of lanolin, a non irritant compound, on IL-1α release. Even at the concentration of 300 pg/ml no IL-1α release could be detected in the conditioned media. We have previously found a good rank correlation between in vitro surfactants induced IL-1α release and in vivo Draize test. We now extend the possibility to use IL-1α production for preliminary screening of chemicals for their irritative/allergenic potential, reducing the need for in vivo models.
In vitro keratinocytes responses to chemical allergens / E. Corsini, M. Marinovich, C.L. Galli. - In: BOLLETTINO CHIMICO FARMACEUTICO. - ISSN 0006-6648. - 134:10(1995), pp. 569-573.
|Titolo:||In vitro keratinocytes responses to chemical allergens|
CORSINI, EMANUELA (Primo)
MARINOVICH, MARINA (Secondo)
GALLI, CORRADO LODOVICO (Ultimo)
|Parole Chiave:||allergens; cytokines; in vitro model; keratinocytes|
|Settore Scientifico Disciplinare:||Settore BIO/14 - Farmacologia|
|Data di pubblicazione:||1995|
|Appare nelle tipologie:||01 - Articolo su periodico|