Griseofulvin (GF) treatment was found to increase segregation of the 8AG-sensitive parental type in hybrids produced between HGPRT- and HGPRT+ clonal derivatives of the human heteroploid line EUE. Continued platings in 8AG medium after a period of growth in medium without 8AG revealed the same degree of stability of the character both in the spontaneous and the induced segregants. Induced segregation rate (ISR), i.e., the ratio between the frequency of 8AG-resistant colonies from treated and untreated cells, showed a marked variation among different hybrids.

Spontaneous and griseofulvin-induced segregation for 8-azaguanine resistance in hybrids from a human heteroploid line / L. Larizza, G. Simoni, M. Stefanini, L. de Carli. - In: EXPERIMENTAL CELL RESEARCH. - ISSN 0014-4827. - 120:2(1979 May), pp. 405-9-409. [10.1016/0014-4827(79)90400-2]

Spontaneous and griseofulvin-induced segregation for 8-azaguanine resistance in hybrids from a human heteroploid line

L. Larizza
Primo
;
G. Simoni
Secondo
;
1979

Abstract

Griseofulvin (GF) treatment was found to increase segregation of the 8AG-sensitive parental type in hybrids produced between HGPRT- and HGPRT+ clonal derivatives of the human heteroploid line EUE. Continued platings in 8AG medium after a period of growth in medium without 8AG revealed the same degree of stability of the character both in the spontaneous and the induced segregants. Induced segregation rate (ISR), i.e., the ratio between the frequency of 8AG-resistant colonies from treated and untreated cells, showed a marked variation among different hybrids.
Phenotype; Clone Cells; Chromosomes; Chromosome Deletion; Humans; Hybrid Cells; Griseofulvin; Drug Resistance; Azaguanine; Cell Line
Settore MED/03 - Genetica Medica
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/181626
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