A full-length cDNA clone for human thyroid peroxidase inserted into the mammalian cell expression vector pECE was stably transfected into the rat thyroid cell line FRTL5. Clones expressed immunologically and enzymatically assessed human thyroid peroxidase protein. Methimazole (25 μM) inhibited the thyroid peroxidase activity dose-dependently and this effect was completely antagonised by 100 μM NaI. Ethylenethiourea, metabolite of dithiocarbamate pesticides, inhibited the enzyme at 50 μM. Thus, we have obtained thyroidal cells stably expressing enzymatically active human thyroid peroxidase which can be pharmacologically modulated and studied.
Cloning of a new FRTL5-derived cell line stably expressing active human thyroid peroxidase / M. Marinovich, M. Guizzetti, T. Zanelli, E. Corsini, C.L. Galli. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - 212:2(1995), pp. 602-608.
|Titolo:||Cloning of a new FRTL5-derived cell line stably expressing active human thyroid peroxidase|
MARINOVICH, MARINA (Primo)
CORSINI, EMANUELA (Penultimo)
GALLI, CORRADO LODOVICO (Ultimo)
|Settore Scientifico Disciplinare:||Settore BIO/14 - Farmacologia|
|Data di pubblicazione:||1995|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1006/bbrc.1995.2012|
|Appare nelle tipologie:||01 - Articolo su periodico|