Spleen cell cultures from immunized and control mice have been followed in their morphological evolution, especially towards "blastoid" and binucleated cells. The immunological activity of cultures from immunized mice has been tested at different ages of culture by the Jerne antibody plaque essay. The ability of spleen cells to synthetize antibody was maintained in vitro for 15 days. DNA and RNA synthesis have been studied by radioautography using thymidine and uridine as precursors, respectively. No differences in the kinetic of synthesis appeared by relating the two types of cultures.

[In vitro cell cultures of the spleen of the mouse. Antibody response and synthesis of nucleic acids] / L. Larizza. - In: ZEITSCHRIFT FUR ZELLFORSCHUNG UND MIKROSKOPISCHE ANATOMIE (1948). - ISSN 0340-0336. - 98:3(1969), pp. 364-77-377.

[In vitro cell cultures of the spleen of the mouse. Antibody response and synthesis of nucleic acids]

L. Larizza
Primo
1969

Abstract

Spleen cell cultures from immunized and control mice have been followed in their morphological evolution, especially towards "blastoid" and binucleated cells. The immunological activity of cultures from immunized mice has been tested at different ages of culture by the Jerne antibody plaque essay. The ability of spleen cells to synthetize antibody was maintained in vitro for 15 days. DNA and RNA synthesis have been studied by radioautography using thymidine and uridine as precursors, respectively. No differences in the kinetic of synthesis appeared by relating the two types of cultures.
Animals; Culture Techniques; RNA; DNA; Spleen; Antibody Formation; Mice; Uridine; Autoradiography; Time Factors; Thymidine
Settore MED/03 - Genetica Medica
Settore BIO/13 - Biologia Applicata
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/181565
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