We have investigated the relevance of actin rearrangement to neurotransmitter release, in neuroblastoma cell line SH-5Y5Y stimulated with carbachol (1 mM). Carbachol reversibly polymerizes actin and releases norepinephrine in undifferentiated SH-5Y5Y cells as well as in tetradecanoylphorbol 13-acetate (16 nM) and retinoic acid (5 μM) differentiated SH-5Y5Y cells. Microscopic analysis of F-actin distribution indicates polymerization of cortical actin. Prior treatment with iota toxin E from Clostridium perfringens inhibits both carbachol-induced actin polymerization and norepinephrine release, slightly affecting the basal actin network. These data suggest that actin polymerization is associated with norepinephrine release.
Is actin polymerization relevant to neurosecretion? A study on neuroblastoma cells / B. Viviani, C.L. Galli, M. Marinovich. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - 223:3(1996), pp. 712-717.
|Titolo:||Is actin polymerization relevant to neurosecretion? A study on neuroblastoma cells|
VIVIANI, BARBARA (Primo)
GALLI, CORRADO LODOVICO (Secondo)
MARINOVICH, MARINA (Ultimo)
|Settore Scientifico Disciplinare:||Settore BIO/14 - Farmacologia|
|Data di pubblicazione:||1996|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1006/bbrc.1996.0960|
|Appare nelle tipologie:||01 - Articolo su periodico|