Protein expression during the early development of Xenopus has been followed by 2D-polyacrylamide gel electrophoresis (PAGE). The analysis of two-dimensional maps of eggs and embryos at different stages of development has allowed the separation of more than 2000 spots. Identification of numerous polypeptides was obtained in four different ways: (1) immunoblotting; (2) amino terminal sequence after blotting on to PVDF membranes; (3) comigration; and (4) assignment in comparison with proteins separated by 2D techniques on reference maps such as human liver, red blood cells, plasma and cerebrospinal fluid reported in the Swiss 2D-PAGE Data Base. The maps presented in this report are a step toward the study of the protein expression in Xenopus eggs and embryos and may be a powerful working tool since Xenopus embryos are popular models for the study of development.
Two-dimensional protein maps of Xenopus eggs and embryos at different developmental stages / G. Tedeschi, G. Bernardini, M. Mortarino, A. Negri, F. Ceciliani, S. Ronchi. - In: CELL BIOLOGY INTERNATIONAL. - ISSN 1065-6995. - 22:7-8(1998), pp. 517-525.
Two-dimensional protein maps of Xenopus eggs and embryos at different developmental stages
G. TedeschiPrimo
;M. Mortarino;A. Negri;F. CecilianiPenultimo
;S. RonchiUltimo
1998
Abstract
Protein expression during the early development of Xenopus has been followed by 2D-polyacrylamide gel electrophoresis (PAGE). The analysis of two-dimensional maps of eggs and embryos at different stages of development has allowed the separation of more than 2000 spots. Identification of numerous polypeptides was obtained in four different ways: (1) immunoblotting; (2) amino terminal sequence after blotting on to PVDF membranes; (3) comigration; and (4) assignment in comparison with proteins separated by 2D techniques on reference maps such as human liver, red blood cells, plasma and cerebrospinal fluid reported in the Swiss 2D-PAGE Data Base. The maps presented in this report are a step toward the study of the protein expression in Xenopus eggs and embryos and may be a powerful working tool since Xenopus embryos are popular models for the study of development.Pubblicazioni consigliate
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