A two-dimensional polyacrylamide gel electrophoresis map of bull seminal plasma proteins has been established. About 250 spots were detected after silver staining and polypeptides from 24 spots have been N-terminally sequenced. Major proteins already described in bull seminal plasma, like PDC-109 and aSFP, have been located on the map; proteins not yet reported in male reproductive tracts have been evidenced; for some polypeptides showing a previously unknown N-terminal sequence, structural similarities with proteins described in other organisms have been found. A reference map of seminal plasma proteins could be useful in relating protein pattern changes to physiopathological events influencing the reproductive sphere.
Two-dimensional polyacrylamide gel electrophoresis map of bull seminal plasma proteins / M. Mortarino, G. Tedeschi, A. Negri, F. Ceciliani, L. Gottardi, G. Maffeo, S. Ronchi. - In: ELECTROPHORESIS. - ISSN 0173-0835. - 5:5(1998), pp. 797-801.
Titolo: | Two-dimensional polyacrylamide gel electrophoresis map of bull seminal plasma proteins | |
Autori: | MORTARINO, MICHELE (Primo) TEDESCHI, GABRIELLA (Secondo) MAFFEO, GIOVANNI FRANCESCO (Penultimo) RONCHI, SEVERINO (Ultimo) | |
Settore Scientifico Disciplinare: | Settore BIO/10 - Biochimica | |
Data di pubblicazione: | 1998 | |
Rivista: | ||
Tipologia: | Article (author) | |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1002/elps.1150190532 | |
Appare nelle tipologie: | 01 - Articolo su periodico |