In order to develop a new derivatization procedure for the gas chromatographic analysis of sugars, O-(2,3,4.5.6-pentafluorobenzyl)hydroxylamine was reacted with the neutral monosaccharides most commonly present in glycoproteins. The resulting oximes were subsequently acetylated, and the resulting volatile derivatives can be separated on both packed and capillary columns. The method appeared to be rapid, simple and reliable. As an application, the neutral sugars content of some known glycoproteins was analyzed.
Gas Chromatographic analysis of neutral monosaccharides as their O-pentafluorobenzyloxime acetates / P.A. Biondi, F. Manca, A. Negri, C.L. Secchi, M. Montana. - In: JOURNAL OF CHROMATOGRAPHY A. - ISSN 0021-9673. - 411:C(1987 Dec 18), pp. 275-284.
Gas Chromatographic analysis of neutral monosaccharides as their O-pentafluorobenzyloxime acetates
P.A. BiondiPrimo
;A. Negri;C.L. SecchiPenultimo
;
1987
Abstract
In order to develop a new derivatization procedure for the gas chromatographic analysis of sugars, O-(2,3,4.5.6-pentafluorobenzyl)hydroxylamine was reacted with the neutral monosaccharides most commonly present in glycoproteins. The resulting oximes were subsequently acetylated, and the resulting volatile derivatives can be separated on both packed and capillary columns. The method appeared to be rapid, simple and reliable. As an application, the neutral sugars content of some known glycoproteins was analyzed.
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