Purging procedures are increasingly used to provide stem cell collections devoid of contaminating tumor cells. In follicle center lymphoma (FCL), most approaches eradicate polymerase chain reaction (PCR);-detectable disease in only a fraction of harvests undergoing ex vivo manipulation. In this study we evaluated whether there is a relationship between tumor burden of stem cell harvests and successful clearance of PCR-detectable disease following ex vivo manipulation.

A validated real-time quantitative PCR approach shows a correlation between tumor burden and successful ex vivo purging in follicular lymphoma patients / M. Ladetto, S. Sametti, J. W. Donovan, D. Ferrero, M. Astolfi, M. Mitterer, I. Ricca, D. Drandi, P. Corradini, P. Coser, A. Pileri, J. G. Gribben, C. Tarella. - In: EXPERIMENTAL HEMATOLOGY. - ISSN 0301-472X. - 29:2(2001 Feb), pp. 183-93-193. [10.1016/S0301-472X(00)00651-2]

A validated real-time quantitative PCR approach shows a correlation between tumor burden and successful ex vivo purging in follicular lymphoma patients

P. Corradini;C. Tarella
2001-02

Abstract

Purging procedures are increasingly used to provide stem cell collections devoid of contaminating tumor cells. In follicle center lymphoma (FCL), most approaches eradicate polymerase chain reaction (PCR);-detectable disease in only a fraction of harvests undergoing ex vivo manipulation. In this study we evaluated whether there is a relationship between tumor burden of stem cell harvests and successful clearance of PCR-detectable disease following ex vivo manipulation.
Sensitivity and Specificity; Glyceraldehyde-3-Phosphate Dehydrogenases; Neoplasm, Residual; Reproducibility of Results; Hematopoietic Stem Cells; Humans; Lymphoma, Follicular; Gene Rearrangement; Transplantation, Autologous; Translocation, Genetic; Proto-Oncogene Proteins c-bcl-2; Polymerase Chain Reaction; Base Sequence; Blood Component Removal; Adult; Hematopoietic Stem Cell Transplantation; Molecular Sequence Data; Middle Aged; Adolescent
Settore MED/15 - Malattie del Sangue
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/181116
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