In southern Italy, 44 contacts of hepatitis B virus carriers, including infants of carrier mothers, became HBsAg positive despite passive and active immunisation according to standard protocols. In 32 of these vaccinees infection was confirmed by the presence of additional markers of viral replication. In 1 infant, serious disease occurred. The virus from this patient is an escape mutant with a different sequence from that of the isolate from the mother. A point mutation from guanosine to adenosine at nucleotide position 587 resulted in an aminoacid substitution from glycine to arginine in the highly antigenic a determinant of HBsAg. This mutation is stable: it is present in an isolate from the child 5 years later. In some of these patients, including this child, the a determinant, to which a large part of the vaccine-induced immunity is directed, has been partly lost. Binding to HBsAg of a monoclonal antibody, previously mapped to the region of the mutation, was reduced in the child relative to that of the mother.

Vaccine-induced escape mutant of hepatitis B virus / W.F. CARMAN, A.R. ZANETTI, P. KARAYIANNIS, J. WATERS, G. MANZILLO, E. TANZI, A.J. ZUCKERMAN, H.C. THOMAS. - In: THE LANCET. - ISSN 0140-6736. - 336:8711(1990), pp. 325-329. [10.1016/0140-6736(90)91874-A]

Vaccine-induced escape mutant of hepatitis B virus

A.R. Zanetti
Secondo
;
E. Tanzi;
1990

Abstract

In southern Italy, 44 contacts of hepatitis B virus carriers, including infants of carrier mothers, became HBsAg positive despite passive and active immunisation according to standard protocols. In 32 of these vaccinees infection was confirmed by the presence of additional markers of viral replication. In 1 infant, serious disease occurred. The virus from this patient is an escape mutant with a different sequence from that of the isolate from the mother. A point mutation from guanosine to adenosine at nucleotide position 587 resulted in an aminoacid substitution from glycine to arginine in the highly antigenic a determinant of HBsAg. This mutation is stable: it is present in an isolate from the child 5 years later. In some of these patients, including this child, the a determinant, to which a large part of the vaccine-induced immunity is directed, has been partly lost. Binding to HBsAg of a monoclonal antibody, previously mapped to the region of the mutation, was reduced in the child relative to that of the mother.
Settore MED/42 - Igiene Generale e Applicata
1990
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/181108
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