We have investigated the effect of calcitonin (CT) on adenylate cyclase in membranes from different rat brain areas. Salmon calcitonin (sCT) dose-dependently inhibited the enzyme activity in midbrain, hypothalamus, medulla, pons and caudate nucleus, but was ineffective in adenohypophysis. The inhibitory effect was enhanced by GTP. Comparison of calcitonins of different origin indicated that sCT was the most potent in inhibiting the enzyme in hypothalamic membranes, eel CT (eCT) was slightly less potent, and human CT (hCT) was ineffective. Chronic I.C.V. pretreatment with sCT did not modify the subsequent in vitro sensitivity of adenylate cyclase to sCT. It is concluded that some of CNS actions of CT might involve modulation of intracellular cAMP levels.
|Titolo:||Inhibitory effects of calcitonin on adenylate cyclase activity in different rat brain areas|
GUIDOBONO CAVALCHINI, FRANCESCA (Secondo)
|Parole Chiave:||Animals; Calcitonin; Receptors, Calcitonin; Hypothalamus; Brain; Adenylate Cyclase; Receptors, Cell Surface; Injections, Intraventricular; Guanosine Triphosphate; Rats; Rats, Inbred Strains; Time Factors; Male|
|Settore Scientifico Disciplinare:||Settore BIO/14 - Farmacologia|
|Data di pubblicazione:||8-dic-1986|
|Digital Object Identifier (DOI):||10.1016/0024-3205(86)90404-2|
|Appare nelle tipologie:||01 - Articolo su periodico|